	   SnapGene      '¼tgcatgcctgcaggtcgagatccgggatcgaagaaatgatggtaaatgaaataggaaatcaaggagcatgaaggcaaaagacaaatataagggtcgaacgaaaaataaagtgaaaagtgttgatatgatgtatttggctttgcggcgccgaaaaaacgagtttacgcaattgcacaatcatgctgactctgtggcggacccgcgctcttgccggcccggcgataacgctgggcgtgaggctgtgcccggcggagttttttgcgcctgcattttccaaggtttaccctgcgctaaggggcgagattggagaagcaataagaatgccggttggggttgcgatgatgacgaccacgacaactggtgtcattatttaagttgccgaaagaacctgagtgcatttgcaacatgagtatactagaagaatgagccaagacttgcgagacgcgagtttgccggtggtgcgaacaatagagcgaccatgaccttgaaggtgagacgcgcataaccgctagagtactttgaagaggaaacagcaatagggttgctaccagtataaatagacaggtacatacaacactggaaatggttgtctgtttgagtacgctttcaattcatttgggtgtgcactttattatgttacaatatggaagggaactttacacttctcctatgcacatatattaattaaagtccaatgctagtagagaaggggggtaacacccctccgcgctcttttccgatttttttctaaaccgtggaatatttcggatatccttttgttgtttccgggtgtacaatatggacttcctcttttctggcaaccaaacccatacatcgggattcctataataccttcgttggtctccctaacatgtaggtggcggaggggagatatacaatagaacagataccagacaagacataatgggctaaacaagactacaccaattacactgcctcattgatggtggtacataacgaactaatactgtagccctagacttgatagccatcatcatatcgaagtttcactaccctttttccatttgccatctattgaagtaataataggcgcatgcaacttcttttctttttttttcttttctctctcccccgttgttgtctcaccatatccgcaatgacaaaaaaaatgatggaagacactaaaggaaaaaattaacgacaaagacagcaccaacagatgtcgttgttccagagctgatgaggggtatctcgaagcacacgaaactttttccttccttcattcacgcacactactctctaatgagcaacggtatacggccttccttccagttacttgaatttgaaataaaaaaaagtttgctgtcttgctatcaagtataaatagacctgcaattattaatcttttgtttcctcgtcattgttctcgttccctttcttccttgtttctttttctgcacaatatttcaagctataccaagcatacaatcaactccaagctttgcaaagatggataaagcggaattaattcccgagcctccaaaaaagaagagaaaggtcgaattgggtaccgccgccaattttaatcaaagtgggaatattgctgatagctcattgtccttcactttcactaacagtagcaacggtccgaacctcataacaactcaaacaaattctcaagcgctttcacaaccaattgcctcctctaacgttcatgataacttcatgaataatgaaatcacggctagtaaaattgatgatggtaataattcaaaaccactgtcacctggttggacggaccaaactgcgtataacgcgtttggaatcactacagggatgtttaataccactacaatggatgatgtatataactatctattcgatgatgaagataccccaccaaacccaaaaaaagagatctttaatacgactcactatagggcgagcgccgccatggagtacccatacgacgtaccagattacgctcatatggccatggaggccagtgaattcatgtcgggattcgacgatcctggcattttctacagcgacagcttcgggggcgacgcccaggccgacgaggggcaggcccgcaaatcgcagctgcagaggcgcttcaaggagttcctgcggcagtaccgagtgggcaccgaccgcacgggcttcaccttcaaatacagggatgaactcaagcggcattacaacctgggggagtactggattgaggtggagatggaggatctggccagctttgatgaggacctggccgactacttgtacaagcagccagccgagcacctgcagctgctggaggaagctgccaaggaggtagctgatgaggtgacccggccccggccttctggggaggaggtgctccaggacatccaggtcatgctcaagtcggacgccagcccttccagcattcgtagcctgaagtcggacatgatgtcacacctggtgaagatccctggcatcatcatcgcggcctctgcggtccgtgccaaggccacccgcatctctatccagtgccgcagctgccgcaacaccctcaccaacattgccatgcgccctggcctcgagggctatgccctgcccaggaagtgcaacacagatcaggctgggcgccccaaatgcccattggacccgtacttcatcatgcccgacaaatgcaaatgcgtggacttccagaccctgaagctgcaggagctgcctgatgcagtcccccacggggagatgcccagacacatgcagctctactgcgacaggtacctgtgtgacaaggtcgtccctgggaacagggttaccatcatgggcatctactccatcaagaagtttggcctgactaccagcaggggccgtgacagggtgggcgtgggcatccgaagctcctacatccgtgtcctgggcatccaggtggacacagatggctctggccgcagctttgctggggccgtgagcccccaggaggaggaggagttccgtcgcctggctgccctcccaaatgtctatgaggtcatctccaagagcatcgccccctccatctttgggggcacagacatgaagaaggccattgcctgcctgctctttgggggctcccgaaagaggctccctgatggacttactcgccgaggagacatcaacctgctgatgctaggggaccctgggacagccaagtcccagcttctgaagtttgtggagaagtgttctcccattggggtatacacgtctgggaaaggcagcagcgcagctggactgacagcctcggtgatgagggacccttcgtcccggaatttcatcatggagggcggagccatggtcctggccgatggtggggtcgtctgtattgacgagtttgacaagatgcgagaagatgaccgtgtggcaatccacgaagccatggagcagcagaccatctctatcgccaaggctgggatcaccaccaccctgaactcccgctgctccgtcctggctgctgccaactcagtgttcggccgctgggatgagacgaagggggaggacaacattgacttcatgcccaccatcttgtcgcgcttcgacatgatcttcatcgtcaaggatgagcacaatgaggagagggatgtgatgctggccaagcatgtcatcactctgcacgtgagcgcactgacacagacacaggctgtggagggcgagattgacctggccaagctgaagaagtttattgcctactgccgagtgaagtgtggcccccggctgtcagcagaggctgcagagaaactgaagaaccgctacatcatcatgcggagcggggcccgtcagcacgagagggacagtgaccgccgctccagcatccccatcactgtgcggcagctggaggccattgtgcgcatcgcggaagccctcagcaagatgaagctgcagcccttcgccacagaggcagatgtggaggaggccctgcggctcttccaagtgtccacgttggatgctgccttgtccggtaccctgtcaggggtggagggcttcaccagccaggaggaccaggagatgctgagccgcatcgagaagcagctcaagcgccgctttgccattggctcccaggtgtctgagcacagcatcatcaaggacttcaccaagcagaaatacccggagcacgccatccacaaggtgctgcagctcatgctgcggcgcggcgagatccagcatcgcatgcagcgcaaggttctctaccgcctcaagtgaggatccatcgagctcgagctgcagatgaatcgtagatactgaaaaaccccgcaagttcacttcaactgtgcatcgtgcaccatctcaatttctttcatttatacatcgttttgccttcttttatgtaactatactcctctaagtttcaatcttggccatgtaacctctgatctatagaattttttaaatgactagaattaatgcccatcttttttttggacctaaattcttcatgaaaatatattacgagggcttattcagaagctttggacttcttcgccagaggtttggtcaagtctccaatcaaggttgtcggcttgtctaccttgccagaaatttacgaaaagatggaaaagggtcaaatcgttggtagatacgttgttgacacttctaaataagcgaatttcttatgatttatgatttttattattaaataagttataaaaaaaataagtgtatacaaattttaaagtgactcttaggttttaaaacgaaaattcttattcttgagtaactctttcctgtaggtcaggttgctttctcaggtatagcatgaggtcgctcttattgaccacacctctaccggccggtcgaaattcccctaccctatgaacatattccattttgtaatttcgtgtcgtttctattatgaatttcatttataaagtttatgtacaaatatcataaaaaaagagaatctttttaagcaaggattttcttaacttcttcggcgacagcatcaccgacttcggtggtactgttggaaccacctaaatcaccagttctgatacctgcatccaaaacctttttaactgcatcttcaatggccttaccttcttcaggcaagttcaatgacaatttcaacatcattgcagcagacaagatagtggcgatagggttgaccttattctttggcaaatctggagcagaaccgtggcatggttcgtacaaaccaaatgcggtgttcttgtctggcaaagaggccaaggacgcagatggcaacaaacccaaggaacctgggataacggaggcttcatcggagatgatatcaccaaacatgttgctggtgattataataccatttaggtgggttgggttcttaactaggatcatggcggcagaatcaatcaattgatgttgaaccttcaatgtaggaaattcgttcttgatggtttcctccacagtttttctccataatcttgaagaggccaaaacattagctttatccaaggaccaaataggcaatggtggctcatgttgtagggccatgaaagcggccattcttgtgattctttgcacttctggaacggtgtattgttcactatcccaagcgacaccatcaccatcgtcttcctttctcttaccaaagtaaatacctcccactaattctctgacaacaacgaagtcagtacctttagcaaattgtggcttgattggagataagtctaaaagagagtcggatgcaaagttacatggtcttaagttggcgtacaattgaagttctttacggatttttagtaaaccttgttcaggtctaacactacctgtaccccatttaggaccacccacagcacctaacaaaacggcatcaaccttcttggaggcttccagcgcctcatctggaagtgggacacctgtagcgtcgatagcagcaccaccaattaaatgattttcgaaatcgaacttgacattggaacgaacatcagaaatagctttaagaaccttaatggcttcggctgtgatttcttgaccaacgtggtcacctggcaaaacgacgatcttcttaggggcagacattagaatggtatatccttgaaatatatatatatattgctgaaatgtaaaaggtaagaaaagttagaaagtaagacgattgctaaccacctattggaaaaaacaataggtccttaaataatattgtcaacttcaagtattgtgatgcaagcatttagtcatgaacgcttctctattctatatgaaaagccggttccggcgctctcacctttcctttttctcccaatttttcagttgaaaaaggtatatgcgtcaggcgacctctgaaattaacaaaaaatttccagtcatcgaatttgattctgtgcgatagcgcccctgtgtgttctcgttatgttgaggaaaaaaataatggttgctaagagattcgaactcttgcatcttacgatacctgagtattcccacagttggggatctcgactctagctagaggatcaattcgtaatcatggtcatagctgtttcctgtgtgaaattgttatccgctcacaattccacacaacatacgagccggaagcataaagtgtaaagcctggggtgcctaatgagtgagctaactcacattaattgcgttgcgctcactgcccgctttccagtcgggaaacctgtcgtgccagctgataacttcgtataatgtatgctatacgaagttattaggtctgaagaggagtttacgtccagccaagctagcttggctgcaggtcgagcggccgcgatccggaacccttaatataacttcgtataatgtatgctatacgaagttatcagctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtattgggcgctcttccgcttcctcgctcactgactcgctgcgctcggtcgttcggctgcggcgagcggtatcagctcactcaaaggcggtaatacggttatccacagaatcaggggataacgcaggaaagaacatgtgagcaaaaggccagcaaaaggccaggaaccgtaaaaaggccgcgttgctggcgtttttccataggctccgcccccctgacgagcatcacaaaaatcgacgctcaagtcagaggtggcgaaacccgacaggactataaagataccaggcgtttccccctggaagctccctcgtgcgctctcctgttccgaccctgccgcttaccggatacctgtccgcctttctcccttcgggaagcgtggcgctttctcatagctcacgctgtaggtatctcagttcggtgtaggtcgttcgctccaagctgggctgtgtgcacgaaccccccgttcagcccgaccgctgcgccttatccggtaactatcgtcttgagtccaacccggtaagacacgacttatcgccactggcagcagccactggtaacaggattagcagagcgaggtatgtaggcggtgctacagagttcttgaagtggtggcctaactacggctacactagaaggacagtatttggtatctgcgctctgctgaagccagttaccttcggaaaaagagttggtagctcttgatccggcaaacaaaccaccgctggtagcggtggtttttttgtttgcaagcagcagattacgcgcagaaaaaaaggatctcaagaagatcctttgatcttttctacggggtctgacgctcagtggaacgaaaactcacgttaagggattttggtcatgagattatcaaaaaggatcttcacctagatccttttaaattaaaaatgaagttttaaatcaatctaaagtatatatgagtaaacttggtctgacagttaccaatgcttaatcagtgaggcacctatctcagcgatctgtctatttcgttcatccatagttgcctgactccccgtcgtgtagataactacgatacgggagggcttaccatctggccccagtgctgcaatgataccgcgagacccacgctcaccggctccagatttatcagcaataaaccagccagccggaagggccgagcgcagaagtggtcctgcaactttatccgcctccatccagtctattaattgttgccgggaagctagagtaagtagttcgccagttaatagtttgcgcaacgttgttgccattgctacaggcatcgtggtgtcacgctcgtcgtttggtatggcttcattcagctccggttcccaacgatcaaggcgagttacatgatcccccatgttgtgcaaaaaagcggttagctccttcggtcctccgatcgttgtcagaagtaagttggccgcagtgttatcactcatggttatggcagcactgcataattctcttactgtcatgccatccgtaagatgcttttctgtgactggtgagtactcaaccaagtcattctgagaatagtgtatgcggcgaccgagttgctcttgcccggcgtcaatacgggataataccgcgccacatagcagaactttaaaagtgctcatcattggaaaacgttcttcggggcgaaaactctcaaggatcttaccgctgttgagatccagttcgatgtaacccactcgtgcacccaactgatcttcagcatcttttactttcaccagcgtttctgggtgagcaaaaacaggaaggcaaaatgccgcaaaaaagggaataagggcgacacggaaatgttgaatactcatactcttcctttttcaatattattgaagcatttatcagggttattgtctcatgagcggatacatatttgaatgtatttagaaaaataaacaaataggggttccgcgcacatttccccgaaaagtgccacctgacgtctaagaaaccattattatcatgacattaacctataaaaataggcgtatcacgaggccctttcgtctcgcgcgtttcggtgatgacggtgaaaacctctgacacatgcagctcccggagacggtcacagcttgtctgtaagcggatgccgggagcagacaagcccgtcagggcgcgtcagcgggtgttggcgggtgtcggggctggcttaactatgcggcatcagagcagattgtactgagagtgcaccataacgcatttaagcataaacacgcactatgccgttcttctcatgtatatatatatacaggcaacacgcagatataggtgcgacgtgaacagtgagctgtatgtgcgcagctcgcgttgcattttcggaagcgctcgttttcggaaacgctttgaagttcctattccgaagttcctattctctagctagaaagtataggaacttcagagcgcttttgaaaaccaaaagcgctctgaagacgcactttcaaaaaaccaaaaacgcaccggactgtaacgagctactaaaatattgcgaataccgcttccacaaacattgctcaaaagtatctctttgctatatatctctgtgctatatccctatataacctacccatccacctttcgctccttgaacttgcatctaaactcgacctctacattttttatgtttatctctagtattactctttagacaaaaaaattgtagtaagaactattcatagagtgaatcgaaaacaatacgaaaatgtaaacatttcctatacgtagtatatagagacaaaatagaagaaaccgttcataattttctgaccaatgaagaatcatcaacgctatcactttctgttcacaaagtatgcgcaatccacatcggtatagaatataatcggggatgcctttatcttgaaaaaatgcacccgcagcttcgctagtaatcagtaaacgcgggaagtggagtcaggctttttttatggaagagaaaatagacaccaaagtagccttcttctaaccttaacggacctacagtgcaaaaagttatcaagagactgcattatagagcgcacaaaggagaaaaaaagtaatctaagatgctttgttagaaaaatagcgctctcgggatgcatttttgtagaacaaaaaagaagtatagattctttgttggtaaaatagcgctctcgcgttgcatttctgttctgtaaaaatgcagctcagattctttgtttgaaaaattagcgctctcgcgttgcatttttgttttacaaaaatgaagcacagattcttcgttggtaaaatagcgctttcgcgttgcatttctgttctgtaaaaatgcagctcagattctttgtttgaaaaattagcgctctcgcgttgcatttttgttctacaaaatgaagcacagatgcttcgttgct  Uy   %   %      !   1   .   #      '             (   "   &   +   5   &      &   D   @      "   6               0          $                %   )      3      )   )                $          0   7   -   2   "   8      +   8   #   0          &   0   %   )   &   5   /      #   %   .      &   9   4      -   5   >   -   8   �   K   .   .   =   2   !      )   (   +   %   <   !   1   (   2   /   +   .   %   6   %      :   +   #      $         )   5   '            %         6   3   !   !   -   '   .   '   <   ,   $      '   ,       $   9   *   .                  1   (   '      ;   ,   %      $      )   )   .   ,   5      '   ,      4   -   Y   C   )   4   K   /         &      %   1   ;   +   *   #   H   -      .   %               #   !   %               &         $   4      (   /      ?   7   @   -   $   1      3   A      %   "   /   1   )   (   1         &      #      E   F      .   3   '            )   /   5   .   "   5      0             "            % &IÄÅÆuÉøù�Ž'	•
pÓÔýþ?{ð£¤¸�bƒ¹õ "õ"¯%%  ‰Dv'Ðq�(	–
Ô
@HŠµS–¥ùËbÃ¿„Þ®M¸ …!Ä"°%é%’& Z JiÇÊÛ
|ñ‚Ëç¨­€º_!Í!"ä"î"! å 'ž�úN]
)
è
!'EqÕÿÔ?¹‰aVc™K²ö �!Ø"ö"1 > ú 1rw‘»6�¢¨ó	)	,	|
—
�„‡Š'hÑØ4¦ô<³ùAD†˜¡
jv§…¯¢"Å"ð%b&. 5 B…Šôƒ�Þ(ö
Õ
‹ÄÅb[é½É¾iÀ86¼9Ê
Nç n!†!–!¦#¶#í#ê%&#  þEÕ.Ñr˜«T—åúeh.cºLéÂ™ßú¹ !à!½"±%“& Ã  îòÀ8	P	t	=
d
QAIŸ¶©Ì›òAtC6~Äž3$/&' gÆÉéjlÆŸ	/	†	N­íƒÚV(‘¥F„��3Üòl“o `!|"…"å"ï"t#  ( 3ÈÉËÏ	º
}uØˆYsž\×ì&|–»ÑEf¦Ÿ%¿&<' EKHêÜ
R„›I{è©®ú)Ë�ôo»ð/ Î!	"  [ [ZA	ª	Ê
±àò‰Ìv”´*cEÖ+ù%Â9»æBý¨"( � À Ù æ ÷ (o.‚û,*
"Õ@05AàÁ5/iŠšÐéO�¯ÙË— ­ ÷ Ž!Y"Ù"ë"" H 0àAO€“	é
(FÖ�"ò8±ù5MÄ@˜Md®Ta‹5 r!#c#& ‡ í Ÿ¼^
%
[™ Oy6€�OŠÒb¢mIúª(.Ê]L ³è«÷"û"÷%+ Ô µ¾±Æ		¡	¶	ù	arçæ�NyºüG°¡=Wc-x&1Cõ¿ e"5 ë sÇ¼*�©¾ô		*	-	ÿ	‚…ˆ¯%³Ò§ò÷=‹Éú6BE‡™f_‚’;k¨¼ˆ‹Ï_°c"&  +2¶ì¿xüh£˜
(i›õC”Ç¢´8wÔ†ìØˆ !£"#@%&I&c& � û †—½ÿT’7J‘‹ndó‹‹å@8VÎr ž #%ñ%& ? ¨Ö¿ë…â}
x½Ù5é!´ªáqwd�)ÓnËc!Ü!Æ"#…#ö#=$W&D  E 4¡æ†Áþ"„ÓùM÷n	‰	
s
ú
/áä_k, WZrœ ¶lá@¾jïþ^½í
)¢O o!À!§#Á#Ï#$K%i%ë%&¬&)'¥'@   / 6 c o • |C‘Ü.þ´Œ–�R´½ÆT¸ê]ò~“£¬Ê!Vs¿@Á“:‘Ïè ú —!÷!"4"ˆ"·#ä# $%%N%À%	&e&‚&þ&{' >¤õ(ß)­ÅÅ‚*øòÈoŽsÿŠ ‡!Ÿ!ì!O$%l%¥%" _ €Î‹…Ó_vÖ
dŸÊc\Û€ø	Û97XËÐ ~#î#°$ÿ$¢&6 % ~ QTÃ€°ÄÒÉÌ/2GJs¥»	z	†
š
X8°ê˜æû <f¤ÂJyÕ÷ù^ "!"¾"²%v&”&­' y Ü ÿzŠìÖ3¢PŠÜzÚãd|ÔžìÔ¨ê ©Ðº á!n# -âF§Ò™E
/”oÿMë4·”ÖÃ†àîï#´&ò&1'o'   jÚ¬Œ	 
UÖ:i»X3Oš¥­ûpÅ× !!<! W¸îïž×þ
Q	ÿ�DU´k~À+"‚"¥"y#$0 O `/‘–t‡�£ï9	u	S
¢
¶
Å
RÜ*J¤ªÑ*0ÍçD½ÕœØÅþhš›G‘!E"’"Ë"ì$B%& ¸ ¯!©çvÊe
 ³ó%uAP´Ÿ �k 4$K&  Ä ZÚàXóUÁ	>
m
B·=IÓœB¡ì7<lÙjÚš Â$b%0&$ ¾ ÇòmÇ%L b
%Ofî :w?„WÞ¦GøÁCŽ
ðómÈ‘î%  m é êÃ	‡	Ã
v®þpÑd?’m2÷>�]i p a!}"†"|#ƒ#% t hkÁ
>n‰uZÛ-â)…ª¬<t4P›”! � æ"ð" ñ ˆÊm0	¯	Ë	
î«²š‚ËÈRÝÆäÛ 0!""!#u#Ž#„$8& ¼iÞR	g	‡ÙsæSÌ#6ÖFg�\ø$6%&Ÿ&% ) É%ÁÐäfÚÀU˜Ÿ°#ví<}—¼:X Z %!·"8$û$%%Ú%á%m&À&â&='_') � ™&ÌOþ5~ú/‰‡tò]õÝäLS”Ø'Ò0§‘ ð!\"T#Š#è#m$ß$9%�% %¯& 4VÊ”¡E“ÒC
»
vZè8rt #3 v FLvI
X[^¶ëbœÕáä|_é‹ªØP2S¹Ì ¤IB‰Gy|á	    !œ!è"›#¼&Ò&'9'O'Ž'µ' {!Ý
#¨´Fòaõ0ÙKgÐñ# 0 Ï!A&~&) Ÿ ý ¿P;n’ý
b]Óö…Jçû5ŽR²pxn¢Y!w"°#Ô$ú&'w') ¹|‚œ9�p‹Sžu¶+¯ñŒC^*®ÆÔ/=aç~_¼î !
"I#Ä#Ò#T%„%Ý&Z'™'  \ ³ÍM	q	Ë
’óÇŸ•µäÑ¼È&Ã:˜Ÿò"  jvãB	}	²à„¿}ŠÍwäFçeˆ,‡ç– N € ° ©"Ô"Þ"ó% L\aB©†öÝ�§1«+dú†¼Cu<Õ!."n"³" ±>[«	Œ
Î
@S¿át\q×ñ� þ$ � Á ø pÜ?~Ÿ	¨	Ö±¶ðM.3&Âa+�‹ÎÕêÚjî• Þ!»"â"ì"#ç%  Ú )O´»ÌØƒíü´
A6öBášjœ1Ñ‘ÍnÖÌ˜ ø �!w#M$ ç ü`
#6Ïc¢’GP°® W!Z"u"Ü"™#Ð&'M'Œ'0 � É ñÖ/~‡-ò	+
—Ê#Â1L²0¢§›¼ÊqŒrÃ "s"Ú"�#ª#ø#$‘%Y&Š&È&'E'„'7 	 !'BØêÐ	y
‰
©
e‘”™7:ˆ‘ó&9¹þ"I|ÏÒGÅØe"¯²È€ƒŽ 6 Í ™"È"#k#’#Î%ê&g'- I ¥ 7�15o]ÍUÙ	
Z
#Nš*²ú6NAñNü +U|Ëø%tæe!s!}!d#“%:&2  A 
Šó1¶á�a	”	¾	ê
)G¿à¯è@E­ŽH�–™ ãmkpŒ> D!Ã!	#-#5#ž#¶$O&—&Õ&'R'‘'" « oŸ§ÖõžPYä×ë%Ãã´wŠÛTb9 2!"##?#$.$È%[&,'¨'8 µ â î Á?0$íðú		Ó	&
v

mš5œPlz}“7T�Žö#i§+yŽ/�2Ñh¹ËL HMn&cÈ!ø"‡# !û¸ G¬½
;¤»Ï‘èWßR…à#?$w$†$w%Ö%+ ˆ ZÈ€ó/_¶hYÌ‹Çco£n+û«)´¥âT´¬a ¯"ü"¼#ú#V$Ñ%ø%y&G'°'8 Ë Ø7­2	G	¬
2\öoÚP¼Ó%s˜J2M©�­¾Þ^�ÀÌA!IÃáé›¶õ£ Ý œ"•#¬#$e$©$Œ&Ê&í&	'j'†'# ’ Ñ ByÃ‰ÇßÍ	Ö	bs"ç�k×¬Ä±&f.DßJ z!Á"F#0 ªðŒ¶¿²ÛýT	±	·	í	èNÛ/J€Oý0”Ì~°>XdÍy'2¨µ,cS Å $"  »í� 	j	¢	ú	‚
•îò¨dl]Ø¢´{„õsöŒ1µ%&  Õ ó á	X	ô	

-
6
O
�
z�Ä»z»)H2¤°cfÀ© f"Ð"& Y %ˆÃtÈ€p 
Ú
>´Ó¢ø>Ê€ˆ`ÊæUaÝ±„!Œ!Ì!"×"0 4 = �5ª¿õ	+	7	
{
€ƒ†‰&¨äóC—š ñg-BZ¹è5}½5�Á˜ù!m!ì#a&%  è2kž		.	@	¹
É
M°ÙQƒšHuz‡“¤b�Õ[ø$Œ%8Ðs#) G ì -½+µ	$
æ�N5Œû7F°<l?©h‰è%0B\®Ø`ŠÊq!X"d"b#ö%& Õþ
¾¾ûg)¼
öŠ“È5�:Ó ‡ŠÊä?U##õ#
%?%&H&V&5  3= £À„øýù
ãjY•žµ )k³Çn¢«þ	�¡ ‰ Ï ö!3"ã#þ$J%M%k%¤%&d&�&¡&«&/  ,y‰¯ÑOi¦¤‹	
™
WD;£9~‰ÁÛÙIxÕ§…¤íŸî] !#m#u&³&ñ&0'n'¬' N ®·í.�•¨æ†¢Wl
¡
Û)<œ/Ôg³šÙ¤"Ê"$ë$A%&J&# l s ‡$ÂKÊ	
mˆotÐYc,>ÝÀsŒïÇ\Å  Ž Ú !" #‚#%í%7&% ˜ %U»$NQÀ“D’e¿çT¯ñô/9W[ s ç#l$Þ$ú$8%Œ%Ù%à%&l&®&. ž ü u¾  :8ü
Œa\�õtµ"1B§Rÿñ`.æ/AJˆ^fmÏ Ÿ !Ã#Ñ#@& °L	p	‹
RŽã
‡»†Œ; ò%& ×Û}†ì}–"µÁKõ`™±Î¡*ÔÌimíV!Ý!º"á"©#÷#
$X&‰&Ç&'D'ƒ'9 @ 6¦ÕµT×ã`	¾“69ê‡Âß¸âçý?�)GÑð‰×Úÿl*o±Ç‚÷8 Ì C!d!Â!Ç"#4#+'§'4 À«Ç6ò#µìù	Ò	u
~
«
1gX›Sõ"»$rxn*1�ÐgÝ¿GHQµô›"®"†#”#ß#>$Ð%ì&i' ©àïìÚÞW	i	yM§Ú.(“«%eze´+Ï"&- < F X $ç‡ÂŽ 4 	´	È
åŽM¡ãðP™É,AY4œZç$/A—ÜøÉ!p!ƒ!‹!`&5  M <~¢­	”§…¡Ð÷úNfŠ	ø
»â	X�ÀØ9¡²Òÿ¦™> £\ 2"$ý$L%j%&G&t&ª&> k r t¯ÿ#9M#K	o	
û
Q`l[sXbœô�+= ´¾â	!0A¿¦®ðrð_…î.Z  ~ Ù  "Â#Ð#æ#k$‹%ì%&?&k&­&- 5Ôª´ÆÚ||Öøt
0-
R!·Àümô(_˜þ$ky¾F*~PìÁ!¹"Î"3#¨#	$*'¦'8 L j q }®� 8ÏÙöÿ"e�¿È<ÿ8^½ ›¥­#Yqxæ#.@¥=–÷-ëØ ‚!"å#$j$Š%&>&_&j&©&� K d e p – — ˜ wxyz{’“”34567óôõÃÚabcd‘’S¾Ç"#$%&OU‡"“Pš¤ËÌÍ"WX|}~ÀÁÂÃ"->?wx;<’“”•Ðõöèéê× é û g!h!u!!€!�!ø!ù!"R"S"‰"$$ $!$"$($)$E$i$ï$ð$ñ$ò$% %E%Á%Â%Ã%
&<&=&f&g&h&i&ƒ&„&¦&§&¨&Â&ä&å&ÿ& '#'$'?'a'b'|'}' 'K ! + 0 7 R f *D|•/StµÄj2
V
\
¥!GTµÐð'5V¹lü"ë;Âóçó”ÝtÄAy#v!˜!ú!"T"F$ó$!%F%Ä%&…&Ã&æ&'%'@'c'~'¡'. ™ í7£ÝpÛ÷[—“PˆS…#W”QÎÂ”W[Ñ<ýê ü i!þ!5"Š"9#¸#$#$*$^$¼$%'%O%ã%. " , ?¥Eµ›q}±+3
W
]
¦ï"/žº#ãCè%p•ÈˆáPpQÍw!™!í!"%f%Å%ç&&'d'¢'= 1 S g 9Ò+€öO�Ø'*0T¢*®Ò qkUñ(š<Ã€ÒÞáÅAïúj‹ ³ ½ ˆ! !·!û!"U"G$P$%G%¦%&†&Ä&A'2 § `ª²–)_àäuŒÅÕÆÆ¾+H¶ù6WºÜóÀÑìÐô<ãJu€�BSžCB ô$Z%''! 8 ¯ f¼¶%ƒû•ÑmýÉÍô%t z$(X%m%•%«%Ü%o& ` š ß Ðš
W™Qg�Þ•@R|ÌvÂë *#¹#+$:$t%ä%,&) ’Ï<Ëd†¤¬NhqÛ	ç	
×
­ËÆP†$Ûh�
Ïy]¾Xj!ÿ!:#f##_$%(%£&( cŒÔ7ÞÀk¥ø;weƒ˜§æh8’µùêÜ”×FúÒ=£'Ñ ý 8! #ï#±$½$ %+ �÷î38`ˆÜ\ˆ0 ”	d‰	T]ÜXž¾Ã:8•Y‘\þz '!6"K"‹"$$$•$P%&&% & FƒœÄ�ÓÍ,�³¹¦æ
YÏÞë2ÃŸK¿øD(&qÉ)©- 3 &< # - C | RU•¦Þr~Å «²Ê0HKt¼	x	­ß±™Ôçð€#P�Èð»z[é
‰qê6ÎÕÚ!C"€"g%''£'!  yg±3Ë{	§ü=â‰ÖäN–·âQÔß#!š!î!R#Œ#Ò$#%1  ³ @�¶3E	ð	
4
M
X
^
‡
›
§
9¹¯g¥)G0$úaæURQ _ “ x!—"¿"#D#�%³%Æ%w&•&è&e'®'( 2 Ó,‚í+U4~‹Ûä=ÕÓŸ/Z†kŒ Ê "V"q#ê#%=%H%¢%&T&‡&±&Å&B'2 V h Ý :õ(M™Î(1Q×f£¯Ó 9ò )ýe˜ßÆoÕßDN	£¡û@ » ‰!ü!P"(#7#“$X%©%/ T z �£¥÷ {ùi£+7±VXÎ}v�ƒ…‡‰‹â_B24¸!æ!V#X#Z#\#o#H$z$|$‰$‘$;%§%+ ›8=P}‹Ù¯¸!Qršl€‹ÌêÝ1{›Äí`ëZð©ëªÑ´ ¾ ¡!§!â!^#Q$%2%. ¨ .Ày-;�Æšê	:
ëxü°Fa ÏžÌÒ‰õ =�¸ÆäôCŸ–D‚ä ½!Ò!#J$b$õ$% mv§«³—`cá¨ƒ,Zí•'äkv›õmÄ‡ï@º!:"="H"á$Q&'€'6 ƒ pŒeãù*¥ãÇ¿È·ú7ÕÝôù0Á Nyì5K��áyðÅ!è!ò!"/# #Ë$+%[%™&×&'T'“'% aGïåvÖCÇÓc	IgX^»ÐòígpÑ•×÷T±C ƒ Ê#Ø#%µ&ó&2'p'  Aûœ	ÁGÝ	±Bn„¯ÐÒs¨õ‹u{ƒ¥Ž %œ%¬%: 9 ° óöJ½·–	™	!
J
ã
õ
-ÌV©¬èêí–JnÉ¼ÊÎ¢›&dÚrò%YôÆ& á É!@"^"#n%q%z%Ý%D&+ Îg?k­À	ì
µÍAÂ×¹ü6 ˜þ¹Yå)›®ü)hq»!=!F!o$~$¸$Ø$»%p&#  kÛ<	�	Æ	+:I&â;jƒ0“xI4øxP¦‚-7ØV !‹$ž$–% Xºð[“
h‚J–òþ·¬¶ ”!ƒ"¦"-&$ a › X^Ñ›ÚtœØ *žÅ]»RßSVµêbí\lVžÍ§Ãa";$% šŸ„`ßA
×wì ,"z#4% £ à ¹ïXó«ÿR	E}ŠÁß™+#=#i#º#$,$u%å%) 0‡¤­Oêðn
·
Ø
UÙK¥-3Î3åE‡ýü›¿âHˆk!¬"`#€#5&.'ª'5 P ­ aÐ:~’¥už ºir†)†ÔT
Æ
	«í3
¾ÖQÙÜ:‘ÐzQ[uY "Ì"\$í$%C%ˆ%]&¤&!'ž'' “6¡?e—Ì:	v	è	¥
8Ý+®Ò§ÎÌÇ‡%&ÿi^™ÓÈ ä!"F"•"`$É$)%š% =’Ì­¶ˆØ÷€	Ü	
£
S‚+1è¥�iÆYœ’!“";#g#& d?¸Ûàù•±
¡'h™ë�¨\“­±«¸; K l 9!#5$L& �ªÕŠùK&<{Ëç&¶�Ík €û¤#A#B$%% °q�”"è8ß$E¦ÎE
f
´ô¢vê9QyúëØG¡>(Ò M!ð#$²$}% ¹ üÁlwxð
f„ìBiÝ•µâÓ‘) v þ ,!£!©!¾$ä$ øïGRV¡ÔZ­M
9æk`Óå› À !0$S$Q%6 [Û"4�¯f©±‰Ýô#X£ä	à

>Y\¿��ù¶¢eq‚�8Ÿ¥º€Ýà=¿«]N¸ÿ{ S!7"%#$%$c%&3 ‚áRYaæd‰Âæ‚	½
1J
C«§h}Š§í^ÝÆêYnŒŸÈ IÂ8mïÄí3d'"L"Œ"–$¢$Ã$'&1&! Å Î9	S]–	º	Þ	?
n
C¸_x•ÃUtƒ;–ÚZ’Û(!4!š$Ê%! ä Hh�
&MŒ&	(
Ó
ç
 &DoÒüGz‰ïR•a§¬Â~˜J^!- 0A„ó‚Ôí
-�º—¡§ò<
c
PgžÐ3;ø@d…@sÄa~¦¼È»Éæ ï%&' ' fÈGZÅÈÎé…	ßìˆ×X�ùE)DrÊ*ºñDenåA’¥. n ¾&;'. † ¿ /n´ß°Å’	ø	 Z`˜å!xMxÑ  ßø4LÀHù4'LN�­ô¾ª 4 ê"ú"' ê …–§ëSÆœ½	w®²šèñ ©àec‘»òŠŽŠë7^q ‡ !b!Û!#„#< . D n å!'-¬³LÄum	ˆ	r
à^cVq›��QÉñÚ\?}’ Ur?ýrì(ž!¿!ë!‡"}#À#ÿ#$¯$%h%¿%ý&('z'¤', $ x } SÂëÃ2¡ÈË1Iº	y	…
7¯Õÿ	y{“Ôönþê3ø“ÓN¬ÏÖ;!~"$ · Vßs	Ä
£þ²)HÒ$æ@ ¼ë×ý;³j¿F�j *"D"�"‘"a% ½ ð lÀ
Â
eý9ví>ªl÷1ö™©«;=OQãh �#ƒ$' € hÝ4Ñùã.rÙåR†qË—uãR"5Õœ� $!ï!¶"#S#‰#7$÷$$%ž&á&^', u Oz	]¸m{‘oŠã^*Š×O‹]­¸4EÓwF{à" !›!ç"Ó$ƒ%}&»&Ü&ù&'Y'v'˜'  iK`²A=âÌ|	?¨Æƒ[p|Üž¦ã0æÇðt• M Ô!²"ñ"$ Œ N³Ëû>§	ñ	_
³
°ïL-%b›¡¦‘»ÍÉpq” #v#�#L$�%9    ª 	‰�nž4\�Ì&é
Y
x
ˆ
¨
�%!H{¬³€•Îvûâ!jS{Ê$å= 1!˜""#�#µ$Ç%9&N&–&Ô&é&'Q'f'�'* ´ ·>F/ï1	F	lõ4:n|h¦*~��ºÎçVKÞ%SÇ„` ¢ Ü d$v$…$x&¯'·'.  Ð ò AxÂº�ü°	Ì	Õ	ì	

5
N
�
œ
œíñºcjkË×1¯ƒ¯ÌÞ‹I~R ¨ y!#"À"E#´% 3 j,=Gtå£¸ç>T|`Ô÷[$7×"W"r#ë# ŸÔý-½ƒåVˆ(ÚmªžTõ!â#ê$	%>%I%£%&U&€& &²& º¿ÜS‡]l� Ý$ù$7%Ø% ßî5S	h	Œ)ïÍÖ 0ÆGhóË Þ#&ˆ&Æ&'C'‚' W #;sóó*'/ ±Ñ˜ÛY [ Š!1"¸"2#ü$s&1 * i )­ì¢Œ2RØÂ Fï!;NþkÁVæ™Üpw=¤",;üÖ ý!Q"8#D$»$&%â%Á&ã&>'`'( ©Nš×&)eã°ÑÔp.Û™Ï$àÇ$‡�à@OoîWBiA ¼ Y%ª%Û%n&' Þ öÊcÏ™Mg˜¤:åg7‘f´g�½x“ÖE
~½ù¢&!J"ÿ")#9$”$%&+& { x‚œ²
¬á Çï`¶åTÓ5¨2 ’ #C#‹#Ñ$Ž% &; U š'¤L|P®ÿ68}ËéW0Šü—u‚„†ˆŠ^ÞêCMY¢13O"U#W#Y#[#]#p#é#y${$ˆ$�$’$1%:%<%¡%¨%S&°&, ‚ ou¦øb¤,Bû`Yf]�ÏìñˆŒÅãóöô•x?¹!¼!Ñ!ç!ñ!<"É#×#I$à$%% újÍI>Å	F~/’wHcÙ÷(w‚¤ñgó,% È!?"]"n$}$Š$×$y%º%C& ¢ Ws›ª\ºÞIéaì	[ñý‰¶¦«µ %3%$ 59}‘>~¬µË¹(ém…Ó7�¦Í2	%9á‡Òã!_#È$4& '�' œÚû#”ÍD
°
ï
gŒë[J u L!¢!¨!#A$ã$) !QŒ°"R•¢F¼
ß
	wYª¬Â|¦œ¹Åémsîªåì2M·Ò¿ !R$™$) … /GgY	ì	;
ÿ
ÑûycÐÿ?r‡Ö`÷«3¥Ç—Cä@¤ å é"½&:'. w äRÁê &,Ç›¹ý±GåbŸêÓÙõý>ü:|T’º‰ëM«!�!¾!ê!¿#þ#®$¾%ü&y', J\_·lz�<ÐdÅo�¥âØ/QkŠö\åõDvEzâ
 Ó!±"#ö$|&�&º&ø&'u'—'5 ‹ © Ï MwŽ²Á=EË.Žë	kìbÊÖ®}ÍŒuŽ¹Íá ¥�JzŠHp}ƒ¡ § œ#K$c$u$´$Ó&'P'�'¶' F¢(îR{_l©öÅòô!é$&'�'' r¨¬˜dâo-:M&6�³.å#Ûwœ?nÚ!VAhÕ I"þ"1#*&, ntwa_Êàeÿ\œîûð–.µGóböv£ðò+$ 1 »!Ç!Ð!;">"Ð$Ö$¹%ÿ%B&R& ¡ |�´"©„Þ
[¹¥¸$Hèä1ˆL¶ÑI !K!â$'4 „ Š �ÀQf+<kÊ¸þ
jÐúcÉÕ>q†×öt‘�uyªão£é!¾#ý#t${&œ&¹&÷&û&'t'x'–'-   qs�n^Éß¸9Lú%�¡-íGQz ñ*Ô J!Æ!ó!0#Ï$Õ$è$¸%þ%'Y þ ÿ  Üäåæç*=BCDEFGLáâj—˜äÔÞ9:;<=8KÖ#$1�Ž	OPí@ALMSTqrst±âc I!±!²!¡#±#ü#Ì$Í$Î$\%ú%û%ü%ý%š&›&'''”'•'C \Ýèú+>HMãoˆ“™¦G‚åÈÀÄ÷ûm�Þèõ2Âü
²î6;BN‚6¶U³ø²zd O!Z!³!"j"x"¢#²#$¥$,%]%%Ø&I'U') ûUDÔõd	¼ŒÎ-{ß>Sîï+Ø ÚIÇ 0>6{`úò !k"y"£#³#¦$Ó%6'J'²'4 ]bìð§BHƒ�¨×È1agG
É¶Ú�öŸ¤ÅøMhö›­·Ãpà�_íObÿ®²@!P!´!ò#$%U%…%K –é,:?IN}‰”šwæ#	[qTÁÛJxìüY_‚é3;vªó,°òhq{³ÒD¢7\C–¯ùè³D e „ ï [!J#M#Ù#^%€%&"&¶&Ù&Þ&ô&3'V'['q'š'/ Þ$HºäèpƒŒæ:\	h
ŽTn¼Ñý®­+<ƒø7U·¡§V´Õ½­!""Å#Ë#Ó#X$-% üBÎöN	r	«� ?Tt,½vÀÈ17ûó z"ó"¤#´#�%­%�&   ] üÑEÕý	Ï²ou¶ÒðöŒÙ
'| !À$%& V´t€e	Ì
½ôÞ…°ÑÓàåï©²!ÉÛJÄÆ;„|™��  .!l"®#7'³' �	“Â HÈ
.C|–«·?¦aq"—#§$Ô%Î&'K'Š'% "
†…À~ix…®å`PfeÛˆ,cÅZkõ³ A!Ê!Õ"ß"#ó#Þ%ô%ï&l'1 : ^K¨¾ nzØ2hzI	ö
.á¾î`¥¼ù‹—Îœ¸ÄËÏáœèîäƒžó&Çx ± !µ!$g$r%E&; ± kc¾ô÷íw9dž©¯ÉC	—	š	H
K
ä
³ÊÍ·Ûª­ë'ÆN÷Êq½L·‰'-è€¯+ O � ß â ¥!Ø!A"P#%V%o%+ dñCI„¸äyb~	â	WéŽ÷ Ko€Ž£G6O—«¯àsÕ ã—' Q!_"ª"{%†%* » M-›®$	¥	^\‡÷Þwÿ2|Õºp—û#½Dr¼f … \!�"´"$�$_%�%Ú&ß&W'\'# ê –@çÃ	®
í
ªDWñ¨íªêe´*v=/"Ü#¹$Æ$Û$#&q&›'H Í Ú(;@JChŠ•lÎøÃØRÂÜý7KyýDZ`kš!4<™«ô-±óir¬¶,E£8Z]ßæŸ‡éK¯ýG ¥ ð 	!>!G!«!K#r$æ$¼% &·&õ&4'r'- —]OQSbÏþ~xÁ	¶rBUº�ƒ´Ó4D°Îú*œiE  !Ö!o"N#Ú#p$$Ù$Ž&Ì&'ˆ'  Óßl\¬	,;�qr,J5¢`ÖòyQ.¸ W "Ì#. ²»Ãð4	=	]	Ç	i
ò
J¸Ê'ÜU]<k’Ò„1®„V¸ùWCŽƒë48!Y$Ÿ$—%% ™%Iåéq�?“Ü;4Aâuîoþ¯yØ=8¨Ð§	!÷ÿ®!Æ#Ô#«$.% _U„çŽ	�
Ï
¡TtÀhãÕ½”ÉFùµÂü“¾Ù"ž"Œ$  CÏ÷\”
·¾‚¸­L· ô Ã"ã"í"ô"®%è%‘& Â ù ýqÝñO	s	Ã×²	iuKh7è8	•!ß!¡"¼"¥#µ#2$.& DÅY©	¬t³U'ƒ(ŒÁÖëó2{•Ûkºïü{"„"§"ž%# Ž Ó Ø ö »@		 	ŽñN/4¡¯@Ãb—-.,wÉÏÿSç– ¬ #!  œ *µ¼ê„Iáþ	$BmÆ³7pv^c(ÒmÎ×Í� b"<$%  ^ b ” {ÍÒýÒÛ^.+Ÿ¼÷S·àñ÷�Úî]’WÎù "Î#N$%% Û PáFÙéâÓW¶�ë2o¨Ä !! Y_Ùœu�Öîý	TÉR
µ
ÐCO›Tk}™ØiŸ™ �!x#Á$ è ñ®	a
$=u±áBÊÇ	š�/!{# ý f	B
†7"ÜK“;Y ["5%& WZµ�›aÒÔàæð³£ÅHQ±<`Ø}x¡ í X!v"H#š#¯#S%Ñ&'8'N'�'´' uÍ
‘…¾õsß¾ªÐd…—ž¯ -"m"Ó"Ý" È ºðÕž	É20~€F‹ÕÂ "r"t"Û"˜#æ%Ï&'L'‹'$ ¤ ò0nô X½	Ï	Ø	d˜$®DM™F�s |!",#>#j#‘#$-$’%Í%Z&4 Ê á  úY×¬.k’Ë£ÙÆ—1L¨³œ¤¬¸½€Ëá@m bÂàš!«#»#ù#$U$¨$v%Õ%‹&É&'F'…' ‘ ˆ‹ S	ó	,
”Ã!IÖ/Ã\}³£bôr§0bÄ ( 
 1o6	?	
#
z
¸
Ù
L4–ŸØy†¯ag#4œ­À‰l!Ë!Ö"a#õ%/ ˆ�®½¥(
k
 
VÚ;.‰’4:ÿ}j€²ÓHÆfý‰Éã„�í Î É"##l#ô#ð&/'m'«' $PÁCÉ	Š
‡ÏsæQ†þº-.ÆI[Ä:e !�#ß%6&? ¥ëñ"´ÙÝëV	_	Ñ	ª
fLW’•š¦8†ÁÞô'’ºæ#>JFÐˆ°ÙÏfÜ)d°³�lö´7 B!U!š"­"à"“#Ï%ë&h'7 ; ¬“¦L©³Å{3{ÕÿJ	³	Ç
÷
ºP
Lâ	QW¿ïûOa›Œ˜+³×@X3—ïý„E}ŸÈ} !Í"F&@ J Q 6vŸÎò!osÙöZ`i1
U
[
¤Ï4†û!7R„!:¼ò’O{å!,vVZôçf!t!~!"$$'$]$h$î$D%‰%&;&^&¥&"'Ÿ'- ¦ ® 8Ñ_´€±p^¡»‹*$î½ú”�¹¿ÌÐâB;âÑIR'PÌù² ¶!%e%”%$ b‘;26«	¿Vj‡‡Ú	æ	
/‚¬¦ÅOÚÝ�?é\{�&uÁy 7!e#s%1  B ² ”Ýf2ŸªŽ¸ÊD	w	ï	L
¦
å
ÞÎÝ1¸Ó®(FO�ˆ¾MZ'	(éÔÞ, P Ù!B"–"Q# ô7¢<˜Íøh·e™°Í._nY…? É ¦!å!'#6#%W%3 l‹d¿îx:â‚b	é	9
êw¯ÿÎÇÔøËŸ&fx jšl�°‚ ã Ä!"9"G".#Ÿ#a$Ê$*%P&˜&Ö&'S'’'A @òõ;	•	˜	›	¿	I
â
ë
ô
*9H´ÀÌ,Ë@ÁÜ¨«áç°éìAm®¸Ï5I‚—Èr¡§š¸Šäq�º6�U à E!
#�$·$p%›% ]Ùò¹Z’
ƒ)Ä_U�ÖÞ˜“!`"<#h#% ¬  ×öŸ…	
¤
TØì,22ä¤†üPXZ˜ûtÇ "”"«"[$‡%™%\&-'©'" >p“·‰øDJß%z&ê¡éx�Ìj¬°áª·( : +!@#$|%/ Í®e¨åWQcå�	¹	Ý	ã	X[^¾�øµ¦Lgp�ž¤‚‹ÇÜßHÁ7_cä3!R!&"$#/$¡$É%&1 ã e@.èÞ‹–¯Äñ%	„	‘	÷	'
Ò
C_n—Fˆ�äQw”L÷ 3`�}»K}MºI¬¹d½m ]!ù") ¶ „Â1
	l	q
„
6]¢b(Uš¨ßŽxÖ‘qñÒü²i'¾EŽ† :!)"�"#$`%( ¼ ï NÜ@á¿
ø8‚ì{©q]pÊ0˜¨3:ÆÒï!NsL g !µ"ˆ#6$‚$‚%Û&à&X']'1  ¹[œú%=èîû¦	Ô	
w
€
²
›ðô3m{~i�$Kj ,z”® iºÌÝo$É#< M& žü¹ä¾<'·SÝ#á#Ü$×% "ëb¡Ö‹L—òEîjÎfŽ¼Ð†’ø+0"C$º$$&r&& ›KH­=A|Ä	
«EèÆë‘ÄéX¡�fw>N"#B#È#Ö#x$‡$�$%0%x%  «ú'èÌ¯
î
6f¥X©«Álàüµ¥†@$˜$Ç$3&œ'# Î ±ãé%D�ÏÄnþ¤­a.ôjü[¤û9{àˆêL?¦ °"­$³$½% dþE[›5¤ï²·-"^Úç>ãu¢UµH 
!ý")&E ‰ [rŽÛ)<AKài‡–ÉF�m·iÏùÈÃÝ8pl€"Œõ,s±ìF:?µ÷©°)b Ó H!N!°!i"½#û#$s$¤$ç$~%·%ù%z&¸&ö&H's'F •#T9çë‹A§ô0`f"	[	F
g
Z»µÍÙ,SŒõ£ÚLwzë:=Rš©¬oßz­¡¬*[ìÙH ¦ÿ5þ­ùñ ?!¬!L#ñ#W$Ò%&!&5'±' Ðsü	ªt±	ª¶¿Åœ-!p"–#­#¿$�&Í&'‰'. ½c8cmyx®yH	á	…½&_»„K¶5N–ª®ßã‚+�ÄÔjâ* w Þ !¤!×!O#f$î&k'3 º Ì Ù'PRýÿ•¤	Â	­
]÷CQVðCj™©µÔ3oÞžÍ"JF ¤ ÿ ª!Ž"Û#$q$€$Å$Ú$å$�&Ë&
'‡'' ˜Ò^TÂï’3	ñ
3 ·ÉsgpÛÔí‘ ÈE_ÁÏûB’ê3·ö �"ª$  Ò × õ CÄ		Â~s®²'-g6çz”RæK¹«  "Â"1$ “ zàé]HSÈàQ
-#èlN˜×h œ "Í# ðY­	A
<t�r½:_–X Ò"G#R%) Ç ùÔmŠW¼	Î	×	cj‘¢ÕØ­Å[˜K²/£EaàólaÔ/ Á !{!"T$Ì%/ #Ž¼¤À³Ü5	>	U	^	È	
j
Ÿ
K~VÝ‘•ž×=±r¯…¹Î(3¬-Ãì 9d!T!5 «³5°ª²ÄñŠõY_þ²	å	0
£¹OK�PVú“… ?Wƒ 29–»á>HzD�OÀ| 6!$&$d%&. Ü;ñg�˜·î	8
á
ó
ËévÜ0?ÀæþÍl·4ÓžYe ¦wX„¹5€ÝT 8"&#œ$" \Þ$V¸	‘
(Z]½1^£fË�…ÞÀûWú©¶^ÝÆ *!%"Z$ $˜%5  ƒZçÝ<ŠÃçk	ƒ	�	ö	

p
ƒ
¾
Ñ
B–ïó2EãavKJzp~_oÉJ§ð9|Åh¹ÑÜî�"(" šJaã5eDÀÅi�Ã…÷eM"Ç#Õ#Ž$—$/%2&0 âSæê†Ž@e!	Z	º+mÙ¬‹£¨îÞ°ëZ !zÙ9>=öþt4¨
ø¯!h"£$¬$¶%&(& Ñ&¼rîb‘”£	à	û	ŸÈ©^ï¨ÿÇµn©Ä2  �"Ä$ Æ Ö ô Xè		»	@
P
Ái}¼—±—^g“•ª "Ÿ"Ñ"Ë%  :U^—
/
7
�
ž
}¢Uu¼åÖ3V•Êv„GúÂÜ¿Ü)!5!›$" Ð`â
…Y	�	ß	õ		
o
Ð
žÇD¹`*Iy¿Ä¦<g¸ÃÛýg"�$  ÏVWT	.
Ž
�
{|ÅÆ{|ä–¾u¥def¶·[\]”ÁÚÛ"  5  qGACNNNNNNGTC,CCAGGCCTGG,GATCCGGATC,CCWGGTACCWGG,CCANNNNNTGG,CCAGGNNNTGG,CCANNNCCTGG,CCAGGNCCTGG,GCACNNNAACGTTNNNGTGC,CACNNNGTG,CCNNNNNNNGG,AACNAACRYGTGCGTGC,GCACGCACRYGTTNGTT,GCACNAACRYGTTNGTGC,AACGCACRYGTGCGTT,GACNNNNNGTC,GAANNNNNNNTTGG,CCAANNNNNNNTTC,CACNNNNGTG,AACACNNNNGTGC,GCACNNNNGTGTT,GCANNNNNNTGC,GAACNNNNNNTCC,GGANNNNNNGTTC,CAGNNNCTG,CCAGGNNCTG,CAGNNCCTGG,CCWGGGCCC,GGGCCCWGG,CCWGGGCCCWGG,AACNNNNNNGTGCACNNNNNNGTT,GACNNNGTC,GAANNNNTTC,CCWGGTACC,GGTACCWGG,CCWGGWCCWGG,ACNNNNGTAYC,GRTACNNNNGT,CCWGGYRCCWGG,GAAGNNNNNNTAC,GTANNNNNNCTTC,AACGGCNNNGTGC,GCACNNNGCCGTT,GCACGGCNNNGTT,AACNNNGCCGTGC,CGANNNNNNTGC,GCANNNNNNTCG,CGATCNNNNTGC,GCANNNNGATCG,TGANNNNNNTCA,TGATCNNNNTCA,TGANNNNGATCA,TGATCNNGATCA,AACCTGCNNGTGC,GCACNNGCAGGTT,GCACCTGCNNGTT,AACNNGCAGGTGC,GCCNNNNNGGC,AACNNNNNNGTGCMC,GKGCACNNNNNNGTT,CCWGGNCCWGG,AACTGGGNNGTGC,GCACNNCCCAGTT,GCACTGGGNNGTT,AACNNCCCAGTGC,GACNNNNGTC,GAGNNNNNCTC,CCWGGTCTC,GAGACCWGG,GATNNNNATC,GATCNNNATC,GATNNNGATC,GATCNNGATC,CCWGGCGCCWGG,ACNNNNNCTCC,GGAGNNNNNGT,AACNNNNNNGTGCAG,CTGCACNNNNNNGTT,GATCATGATC,CCWGGNNCCWGG,AACTGGNNNGTGC,GCACNNNCCAGTT,GCACTGGNNNGTT,AACNNNCCAGTGC,AACNAACCGGTGCGTGC,GCACGCACCGGTTNGTT,GCACNAACCGGTTNGTGC,AACGCACCGGTGCGTT,GCANNNNNTGC,GCACNNNNNNGTTCGAACNNNNNNGTGC,CCTNNNNNAGG,GCNNNNNNNGC,CCANNNNNNTGG,CCAGGNNCCTGG,CCTGGCCAGG,CAANNNNNGTGG,CCACNNNNNTTG,TTTAAACNNNNNNGTGC,GCACNNNNNNGTTTAAA,GCACNNNNNNGTTTAAACNNNNNNGTGC,CCAGGNCCY,RGGNCCTGG,CCWGGGNCCTGG,CCAGGNCCCWGG,AAGNNNNNCTT,GAYNNNNNVTC,GABNNNNNRTC,GAYNNNNNRTC,GATCNNNNVTC,GABNNNNGATC,GAYNNNNGATC,GATCNNNNRTC,GATCNNNGATC,GTYAACNNNNNNGTGC,GCACNNNNNNGTTRAC,GCACNNNNNNGTTAACNNNNNNGTGC,GATCNGATC,TAACTATAACGGTCCTAAGGTAGCGA,TCGCTACCTTAGGACCGTTATAGTTA,CTCTCTTAAGGTAGC,GCTACCTTAAGAGAG,TAGGGATAACAGGGTAAT,ATTACCCTGTTATCCCTA,GTTAACNNNNNNGTGC,GCACNNNNNNGTTAAC,AACNAACGCGTGCGTGC,GCACGCACGCGTTNGTT,GCACNAACGCGTTNGTGC,AACGCACGCGTGCGTT,TCCAACNNNNNNGTGC,GCACNNNNNNGTTGGA,CAYNNNNRTG,AACAYNNNNGTGC,GCACNNNNRTGTT,GTTTAAACNNNNNNGTGC,GCACNNNNNNGTTTAAAC,CCWGGCGCC,GGCGCCWGG,CCWGGNNCC,GGNNCCWGG,GATCGCGATC,AACNAACATGTGCGTGC,GCACGCACATGTTNGTT,GCACNAACATGTTNGTGC,AACGCACATGTGCGTT,TGGCAAACAGCTATTATGGGTATTATGGGT,ACCCATAATACCCATAATAGCTGTTTGCCA,ATCTATGTCGGGTGCGGAGAAAGAGGTAATGAAATGG,CCATTTCATTACCTCTTTCTCCGCACCCGACATAGAT,AACNNGCACGTGCNNGTT,GAACNNNNNCTC,GAGNNNNNGTTC,CCAGGWCCY,RGGWCCTGG,CCWGGGWCCTGG,CCAGGWCCCWGG,CCAGGWCCTGG,TTATAACNNNNNNGTGC,GCACNNNNNNGTTATAA,GCACNNNNNNGTTATAACNNNNNNGTGC,GAACNNNNNNTAC,GTANNNNNNGTTC,GGCCNNNNNGGCC,AACNNACTAGTGC,GCACTAGTNNGTT,AACTAGTNNGTGC,GCACNNACTAGTT,AACNAACTAGTGCGTGC,GCACGCACTAGTTNGTT,GCACNAACTAGTTNGTGC,AACGCACTAGTGCGTT,AACNNNAACGTGCNNGTGC,GCACNNGCACGTTNNNGTT,GCACCCANNNGTT,AACNNNTGGGTGC,TTAACNNNNNNGTGC,GCACNNNNNNGTTAA,CACNNNNNNTCC,GGANNNNNNGTG,CACNNNNGATCC,GGATCNNNNGTG,GATCTAGATC,CCANNNNNNNNNTGG,CCAGGCCT,AGGCCTGG,CCTCGAGG,GATCCGGA,TCCGGATC,GGCGCGCC,GCGATCGC,CCWGGWCC,GGWCCWGG,GATCGATC,GATCATGA,TCATGATC,GCGGCCGC,CCTGGCCR,YGGCCAGG,CCWGGNCC,GGNCCWGG,GGCCGGCC,RTGCGCAY,GATCNNGA,TCNNGATC,CGCGCGCG,CCTGGCCA,TGGCCAGG,CGCCGGCG,GTTTAAAC,GATCGCGA,TCGCGATC,TTAATTAA,VCTCGAGB,CCTGCAGG,CRCCGGYG,CGTCGACG,ATTTAAAT,GCCCGGGC,GATCTAGA,TCTAGATC,CACCTGC,GCAGGTG,CCTNAGG,GATCGAT,ATCGATC,CCTCAGC,GCTGAGG,GCTNAGC,CCTNAGC,GCTNAGG,GCTCTTC,GAAGAGC,GGTNACC,CGGWCCG,ACCWGGT,RGGNCCY,CCTGGAG,CTCCAGG,GGTGATC,GATCACC,GATCNGA,TCNGATC,GGGWCCC,GAAGATC,GATCTTC,CCCWGGG,TCCNGGA,TCCWGGA,RGGWCCY,TTATAA,AGGCCT,GACGTC,GTMKAC,TCCGGA,TGCGCA,ACCTGC,GCAGGT,GGTACC,AGTACT,GGYRCC,CCGCTC,GAGCGG,AACGTT,YGGCCR,RAATTY,CTGAAG,CTTCAG,CACGTG,GRCGYC,AGCGCT,CTTAAG,ACRYGT,ACCGGT,ACTAGT,CACGTC,GACGTG,GWGCWC,GTGCAC,CYCGRG,GGGCCC,ATTAAT,CCTAGG,TTCGAA,GCTAGC,GKGCMC,TGGCCA,GGATCC,GRGCYC,ATCGAT,CACGAG,CTCGTG,GGCGCC,GAAGAC,GTCTTC,GCATGC,GTATCC,GGATAC,TGATCA,ACTGGG,CCCAGT,CTRYAG,RGCGCY,AGATCT,GGNNCC,CTGGAG,CTCCAG,CTTGAG,CTCAAG,CGATCG,GGTCTC,GAGACC,YACGTR,CCNNGG,GAATGC,GCATTC,WCCGGW,GCAATG,CATTGC,RCCGGY,GCGCGC,GAGGAG,CTCCTC,CGGCCG,CCCAGC,GCTGGG,GTGCAG,CTGCAC,CGRYCG,CGTACG,CGTCTC,GAGACG,CCATGG,TCGCGA,GDGCHC,TGTACA,TCATGA,ACATGT,GTATAC,CCWWGG,CTCTTC,GAAGAG,GCNNGC,CCRYGG,GTTAAC,RCATGY,TACGTA,RGATCY,GCGATG,CATCGC,GCAGTG,CACTGC,CCCGGG,CCGCGG,TTTAAA,GGCGGA,TCCGCC,GAGCTC,GATATC,CTGRAG,CTYCAG,CAGCAG,CTGCTG,GAATTC,ATGCAT,CATATG,GTYRAC,AAGCTT,GTNNAC,TCNNGA,CAATTG,ACGCGT,TCCRAC,GTYGGA,GCCGGC,CMGCKG,GCCGAG,CTCGGC,CTCGAG,CTGCAG,CAGCTG,GTCGAC,CTYRAG,AATATT,GATCGA,TCGATC,GACCGA,CACCCA,TCGGTC,TGGGTG,WGTACW,TARCCA,TGGYTA,TCTAGA,GGATC,GATCC,CCWGG,GTCTC,GAGAC,GCWGC,GGNCC,CCSGG,GGTGA,TCACC,GGWCC,GCAGC,GCTGC,CCATC,GATGG,ACGGC,GCCGT,GCNGC,CCNGG,GCATC,GATGC,ACTGG,CCAGT,GGATG,CATCC,CTCAG,CTGAG,GGGAC,GTCCC,ACNGT,CTNAG,CAGTG,CACTG,GACGC,GCGTC,CCCGC,GCGGG,GANTC,CGWCG,TCNGA,CCTTC,GAAGG,GTNAC,GAAGA,TCTTC,GAGTC,GACTC,GTSAC,GAWTC,GCSGC,CASTG,ATGAA,TTCAT,ACGGA,TCCGT,CGCG,CCGC,GCGG,GTAC,AGCT,GCGC,CTAG,GATC,GGCC,CCGG,CATG,RGCY,YATR,ACGT,TGCA,AATT,CCTC,GAGG,TTAA,ASST,TCGA                                                                                                                                                                                                                                                                                                                                                    6                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             	                                                	                                                             #                           !                                               	                                                      	                                                                          %                               	                                                
               0         	                           L   +                                       
   
                     #      	                                             )   $      4   0         +      1   ¡   §      9   2      5   '   š                                                                                                                                                               6                                                                                                                                                                                                                                                                                         	                  	                       #         !                 	                    	                          %           	                  
     0   	         L +             
 
       #  	               ) $  4 0   +  1 ¡ §  9 2  5 ' š   šç  œ¯  œÛ  �3  ¢•  ©�  ²s9	Å
J~Ë"À�	;	k	­		
Ž
<{~�ËèKyz¨ZuÀúû/z(:à÷Ÿ"u	·�ø�&« „ëk
z
W‰³Å
ßÊè N%º
ìþœè#?#þ	™ ”	|ëŽjÑ ¶ú	0'!¯ìÖÖÔ„Ø…y
ˆ"@¨ZÑ Ãüƒ­¶ó$PÛäX	a	Ÿ	¨	Í	Ð	ú	-
v
y
)\ˆ×0‹¹"%@Es>AX«Ï{§"’ÕGƒ‡#�#•#M$È%$ÜžZ$¿À=$k$ Ä ¥u	·Ó:¡Ö�Ú ¥u	%·Ù:f‚kw� ¥u	·:� : ¥ ¥ : ¬ df%é	HØ¨çŸfj�, “!3A[Ë…!¿Uµ/&0
49û	3Àà›+ÃãKÀà›3 KK¨û	s å§!P�	Ü
EY©	æ‚	¿@µ¸FÕd"3	U @µ‰	¢ÖXû<!Ð
D 4	Àž"4	¿@µÕû–+"™~§/5q�#�%Yæ1}	 "º
u ’s � O*
º
u�aØK´)ÓÜ!™vë ´°Å`åŽM ¯b,w (tÅÅGµø5V¹òëŽl	q
]Ušü'$¬?!ñ#� þQ	*
­ +"}©#÷#
$‰&Ç&'D'ƒ'-e‚‡ƒy#mâ0	éáq²RÛ 0!!#m²R0!!#èü	t�&¹§cá,›m@ïØñ mNÕ¹²R0!!#´°M hxÿ¹qÄ`"Å-!� *
„$« 1Eéà!Â Ü|è%èw
�$ zqÊ !à†3&� }†,*
Á±/¡š©#÷#
$‰&Ç&'D'ƒ'i� Ã O	*
=
º
p @Aµ¶Øy¸¹uÒhb[)�86]a¿è»	"ñ÷ 3	ÛêaˆyyH	½_‚�! [Ø3%ð�·Šþ	�	²	õ	
‘
Ð
Ñ
B}#DE©Ü0K|Ö?W_2|¸>a	r5æy¸0$oAËé	êË�a$Ñ BÃ­±&å*	‚…ˆ%òB’íŽ¯,Uâ &
mP“HœTŽ¯³,Ái ™ m"¶ì›£"�·Ü0ñ mNâ0	xÕÿé¹áq²RÛ 0!!#Ñž©NèØ!A"e‡™~/�·þ²	Ü0K?W2Qžª!ùþ&  Ñ 1ƒ²ÛT	îòEü°ÍÑ'L|ø"•#¸'ð�·‘
Ü0 e1¥
E‡•"3% i«‹	9h™¤ú!!O›	ÀA ·¬: 9L Ô À ÷ oÕ`ò.qÿ|ñl	q
]Uš÷ ü'$3	Û¬ê?!ñ#’8†Ð°�î3{$Å•&°äéçÆmŸ ™mb
O~\ž²§ä/éŒUçR:0!!#|#%%„%á% B»Ïçž©Îi
ö
²N`ÿó|º8½5‡éKžèëØ!A"_"¼%E&�&¦ ‹Ð;ÖŽëM	q	b_Ñ "ëÑ	šj‘ôÏfla!à"ß"kü	t %èw
�$ z "ëÑ	šôÏfyyH	ü	½_ta-‚ˆ�!ö¢*á·!G$ š ‚3!Àûh^! ’Ñs #î½.  «Thº™ú! ÚŒ	:i3O¥×!çÝŠÃ5	>	ƒ	�	ö	
Ñ
BK~–EãvK•ž×_J|3¬¹\a�Õ!n"¶ì(›Ø£"@%I&!$ØêíúÐ	Ó	v

©
eš‘”™7lz}Žó#&i¹"+IyÏØh¹Ën¯²€È6 ™"’#Î%ê&g'Ã Ô ZòÀ	ª	=
qA¶ày¹ºG›A6V~Ö¿ýe"/& Ö ô 		Á}“Kª Ÿ"Â"é	†	­‘o `!|"…"däbâ	WŽ÷Ko€£G6 ãQ!Ã ZòÀª	=
A¶à›A6~Öý/&!ØêÐ	©
e‘”™7ó&¹"IÏØ¯²€6 ™"’#Î%ê&g'$íúÓ	v

šlz}Ž#i+yh¹ËnÈñá
ó
Ë?Àæl·4 ¦¹5T œ$% Ã€ÒÌ¥XêÂJ÷º–þsF#� ‰!$?~ØêíúŸ	Í	Ð	Ó	Ö	v

©
beš‘”™7lz}�Ž×ðó#&Mi¬¹Ä"+.3IyÏÂaØ+.h¹ËÎDßn¯²€È 6 • z!™"#’#Î%ê&g' Ö ô çÝŠÃ		5	>	ƒ	�	ö	
Ñ
BK~–EÁãvK}•ž×_J|3¬¹“Kª Ÿ"Â"”	¾	ê
)G¿à¯è@­H�–™ãpŒD!	#¶$†
š
8 f¤ù^ ¾"²%v&”&­'d?•«¸l #ÀÈ	r…¹-Ã9d!.r—åúe½"±%“&;	*9Há‚�6U �$…„+Äjî&k'„¨ßñ† #@HµËÃŒ
Î
S¿ïÛOXàÎë¨\
‚ýò9HK ƒ"¦"€#5$Q%5&"„…¨ßòE„^Ðñ+Äj† 0"#r&î&k'ÁÉ	‡ÏÆ[Ä �#6&>·ß&ê¬°ª·: ¸îþQ	D~À$Ïc® Ü"Æ »	i—±Ë%¶�â"ì"ç%¸ FvÒ‚AÈo”Ös´ÿ‹�%l%ñ%´&ò&1'o'�Öý	‘ÐT}—žx#ßü\N	r	,\ÞiwtÕpûˆÖ7½D  … �"#¤#´#Ì#ó#$_%ï&l'Sæê@e!	Z	º+Ù¨Þ þ4ø&E æ†Áä 
o!{É!ã	}	Ã
Ý
Ñä?f#F}¼0K–Ði %!©"8$ ¡„ùM‰	áWœþ¢$K%i%&ŠØRKy<™«¬ZŸýð >!«!K# &4'‹ñ%¸ vA´�ã	}	Ã
Ñ?F–i ©"FÒ‚Èo”Ösÿ%l%´&ò&1'o'� ‰?~Ÿ	Í	Ö	b�×ðM¬Ä.3Âa+.ÎDß • z!#>·ßÁÉ	‡&Ïê¬°ª·Æ[Ä : �#6&- C «²Ktß€PÈð[
qg%''£'bì�¨ÈG
É¶ÚÅMöp®%U%ðhJ”!-&¬	;�qW È ºðÕž	É20~€F‹ÕÂ "r"t"Û"˜#æ%Ï&'L'‹'Ç ùÔmŠW¼	Î	×	cj‘¢ÕØ­Å[˜K²/£EaàólaÔ/ Á !{!"T$Ì%� Á ø pÜ?~Ÿ	¨	Ö±¶ðM.3&Âa+�‹ÎÕêÚjî• Þ!»"â"ì"#ç%4VÊ”¡E“ÒC
»
vZè8rt #À«Ç6ò#µìù	Ò	u
~
«
1gX›Sõ"»$rxn*1�ÐgÝ¿GHQµô›"®"†#”#ß#>$Ð%ì&i'� É ñÖ/~‡-ò	+
—Ê#Â1L²0¢§›¼ÊqŒrÃ "s"Ú"�#ª#ø#$‘%Y&Š&È&'E'„'žü¹ä¾<'·SÝ#á#Ü$×%  jÚ¬Œ	 
UÖ:i»X3Oš¥­ûpÅ× !!<!Ô µ¾±Æ		¡	¶	ù	arçæ�NyºüG°¡=Wc-x&1Cõ¿ e" Ò × õ CÄ		Â~s®²'-g6çz”RæK¹«  "Â"1$ B ² ”Ýf2ŸªŽ¸ÊD	w	ï	L
¦
å
ÞÎÝ1¸Ó®(FO�ˆ¾MZ'	(éÔÞ, P Ù!B"–"Q#‡ Ô í ©ŸàïÀ«Çì6¼µ¾ò#^±µÆÚÞìù			W	i	¡	¶	Ò	ù	
%
u
~
«
1[agry™ç MX›§Úæ.Oy�6NSy€�ºõü"(GOŠ“«»Ò$rxbn¢°¡=m*IWcúª(.%1ez�Ð-gxÊÝ&1C]¿GLe´õ +H³¿èQ«µô e"›"®"Ï"÷"û"†#”#ß#>$Ð%÷%&ì&i' B U { ² ”šÝô'7x‚œ¢¤<Lf|˜Íø2hP®·eŸªÿ68}Ž™°²¸ÊD	w	ï	
L
¦
å
¬ÞÎÝ1¸Ó®(FËáéOW 0Š�ÇÍïüˆ—¾MZ'u‚„†ˆŠ	^`¶(._nÞåêCMTYY¢Óé135…¨ÔÞ, 2 ? P ’ É ¦!Ù!å!B"O"–"#'#6#C#Q#U#W#Y#[#]#p#‹#é#y${$ˆ$�$’$Ñ$%1%:%<%W%Ž%¡%¨% &S&°&šŸ„`ßA
×wì ,"z#4%   ª 	‰�nž4\�Ì&é
Y
x
ˆ
¨
�%!H{¬³€•Îvûâ!jS{Ê$å= 1!˜""#�#µ$Ç%9&N&–&Ô&é&'Q'f'�'§ `ª²–)_àäuŒÅÕÆÆ¾+H¶ù6WºÜóÀÑìÐô<ãJu€�BSžCB ô$Z%''Ñ&¼rîb‘”£	à	û	ŸÈ©^ï¨ÿÇµn©Ä2  �"Ä$ë sÇ¼*�©¾ô		*	-	ÿ	‚…ˆ¯%³Ò§ò÷=‹Éú6BE‡™f_‚’;k¨¼ˆ‹Ï_°c"r¨¬˜dâo-:M&6�³.å#Ûwœ?nÚ!VAhÕ I"þ"1#*& ‚áè2RkÀY«Ç6ašæŸò#d‰žµÂæìù			.	@	‚	Ò	u
~
«
¹
½
É
1Mg°1X„›JSõ"`»Ù$Qrx
Cnƒš«ßHuz‡“§h}Š§í^¤Ý*ÆêYbnŒŸÈ AI�ÂÕ8[mï1�ÄÐø
$gÝŒ¿í%8GÐ3H×dQwµôì '","L"Œ"›"®"s#z#†#”#ß#>$–$¢$Ã$4%Ð%'&1&ì&i'  ] üÑEÕý	Ï²ou¶ÒðöŒÙ
'| !À$%  6+     Í  "Ì        "Ì���¼Å†	XVA[PM…TufP,\UeHU"†a‰¨:Œ3ÅUÄ©ÍhÝmŽâÌî�Ãs€ÈCˆü0Jª3ãj½B§øÎPF�ÖQcÂ€(Æq÷x"ÚjR�á#j5ØIžEÔz1Ä¨ð‚1u‘1ö¡BÌÙ|äIêA]5�(çÒedt'g]àV
.*s«ZÚ€ˆÞ¦˜§Y�@^§zï˜Ý™¥¥KÖ9$E 	wþó;ªñª«•òš°fú¢Š¼�f¯º®}_ÙÛ¯™gë(.þ]‰0@™ÖEÑŸGQÙ`9]G�õ§xûh`‚v\^YâOäz=¶Ù±J·Ÿê¯j=¹¡Ie�Í�zêºª«ªîïü¢‹·Ù¼ÖUVw•U¥ÇTtß]²ŠôN@&ìSwê¯¯­­ÍÞ{¹a5Âg¯¯Jz¡YUT¨âèæÔ™Y�i¥º¢¯²Ú+²¿«¯¢®ªãušµ9ŸSg[^õ:�Q�•0ZZüO½UEP±h	óÏZ¥µHš8dí¢úÞ­åÒMp¼_¶]{WV»S:·_Z>û—+a—­…–·IV†%–µ_x·à p}^2eÌ¨Þt*Y÷�`a‰™y¹¬a…ÿ}_ÕUº–qíæ@ÄÏ=�'öqÉôiÎÙƒØô…­�ˆ<;ãÌðþ�ÿ<ÝÀsÄó/<Ü8Ò~ Ñ½wo¼ã´|ÜðßâÂk�ÎtøDakûŽ}\ˆ®*�£ˆ*m@ouc•‹¯Pn�XñªO€k½ôEèÒ¦½ Cj€4YÑ�mkAÐ‡×�Q!¡ßmW×øqÑE¶ØŒèõºû†ãïcØþãÒ¹ˆ!t„âøôâ}°Ø°ˆúµÛ=9Ïã·'a£7mÀøSÐ‡©�Þ �Ù©ø[vÄ÷…ZÓ­1ô~¯Õ;A®±T¡haëTáG«F…u¼Û×šU�X8©½±;�[$g…WüÔ­ë^#l�öíjêÚ™Û*�®©‰yžû”«[ ö%û†æEª¥aäZXýºª ~VºØU™§:kE:�ë¬ôB µ.õµ
,:.~�EjqT`UÕ²‚FrŠë•”Å«¦˜j¦šVR™UVR&uj•!î�ªUÅZé®„—º¾$-
:»BdØBÎé¡÷~çÃÂÅkþ˜]šö¨–¬‹*æ˜Z¶¦T©‰Õ›eUD[ª”Ô®—®°ÇM·Ç¬ ž(ß•·Ò¸gãoUú©xÛ­`ú~ºÐÔ­aÖ­vÚ4ÑÔdƒ‡é® Õ¸Eò‚²u}XÂ+¢àÕõ4`×Wõø\ël†mõv(à†™gÚ�€ +¥ä`ÃEµµ¡ŠëX�V²º*ûÝ*®ö[¡D=Wi:›ÔVCX íŠ$aSk¢ê7®Â&ŠÞoÔÇ}·Û.¾®éõIYû÷–»‡]qKIú“Vˆ:e.U,Õ)Ø.” ÉÖ…+©Áª’Wè­—yøŸö¤tß—UÃm~º:ôÏ¶àRß‰2ÆM7ßZV¬VÅèv�qvÑY:”_¥ƒ¬8†®‡× ·àÕqÆë¤Gi	›èVfffŽ‰T%JEIG9}ù U]d/¥eš-zÔš!�M©-…Î»šæaTð¯îß«ê®ýj­(UBf2ÐÇî¥ÕV¯KlZ†¸ŒdÏøˆ®Êb„U–
9Dkº6éÆ~k÷J nÇ¹9mk%¶dâ¯ˆ…h¦ó·Z÷uÙÄðSeH•> �–—·ihÊ3·�Î¯K_‹#Ó†^²e‰Žg¦�¸Q©ËÓÔäè8Ð±0óÁ¥™zÉ–&9œR›N6–Ã×3 D0¨É 3ºó¯³·‡³Œì,¬8Ã	´ííPÂYÂ›ÝÐ\ÐT]ˆMT=5PôÉU2Žª½�ï?þM·Ulsµ-ƒüteF}«ÿ�NþÈÎïŠñþ<éðgâóêïëLƒ:»díÎ$&í+�²³½Nˆ�Åÿò´ü|ã>›Â^lºõü%Ç¦Ïx4ÓÞ	tM˜�Â9Ñ+¡H ùyÃ�äPtš‚n3¸áOJ~°UD “ºÃWWß8$Â
ªŠ�M4iÌÑUPnÔQ¾¡º®p‡;H{r”¨-„iµT­ùê•¥VªVÖåI™„•è.)õŽ–Òß›´Æâæ¬­½’�‡¿Ë"F^qœŸnôŽ5†|Äs·Ã½›Me}=<4Rã^¦óïoKšZ(ðäII+=)d¨ÍrŠ=£�²·;,¨&¶´ï
ýqµJvÿbˆÕTÂ“¾°¾ñ²‹E%(<Ò)·¶
`ÓxÙkºx¶=¼”cªâIí}Kk„Y"c|J;£ðËYÀeŸ3Ý“EêU#¶Ú\®°UîÔ§Î(Fô¬b_ÞÈß×†ëMº§«}2®Ut5XóUPP1ÜŠ{gºú«Y¦…6¦Ð)¢í„Á�š…‰©Y]Y ¯3v¯ñT�~¹E}¦›aÚ_™U�É·þ¬»3*Âp…_¸wcNüÂÝ:.%0cÀMOË@Ì´ÀŠ°—˜ÃmF‰á7ÙsjSew7˜òºí‰™™�w4fB1È]!8˜ŒÓ³(Ú¬ÎÊ¬Î¡Jù¯¯šîNEI�^´Lê…_U3¸QÍêÕWÕ\ß‰q9åY£|ëÝ]£µRnê9™»ˆæoæeùý½ú³¾…èÛ•ì~çj©Š›¹&žêGUV‰%šÙÅ]gbWjùœ’fDuYWÊÙj¸}aE¶×9·«ŠÝRc5½Û	‘f[ cên…U�üÓ¬ä–ÒW0�-ª¦VG}RO®¹~—çHÕR›Q6™7TUR[”c¨¤UdÎì6ª$]UE&FºŠ	VLîÌ£jâ¸•XÓ´k¨¨Ui3»2�ªŠ§UaMÑ®²‚U“:³(Ú¸®%V4íê*ZLîÌ£j¢¹ÕaMÑŽ²Ž      "Ë   -<AlignableSequences trimStringency="Medium"/>  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  (¬<?xml version="1.0"?><Features nextValidID="20"><Feature recentID="19" name="2Î¼ ori" type="rep_origin" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7741-8905" color="#ffff00" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;yeast 2Î¼ plasmid origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="18" name="LEU2" directionality="2" translationMW="38980.99" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="3640-4734" color="#ff7f50" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;S. cerevisiae LEU2&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;yeast auxotrophic marker&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;3-isopropylmalate dehydrogenase, required for leucine biosynthesis&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="17" name="AmpR" directionality="2" translationMW="31557.36" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="7285" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1" detectionMode="exactProteinMatch"><Segment range="6494-7285" color="#ccffcc" type="standard" translated="1"/><Segment name="signal sequence" range="7286-7354" color="#ccffcc" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to ampicillin, carbenicillin, and related antibiotics&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;Î²-lactamase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="16" name="ADH1 promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="771-1476" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;S. cerevisiae ADH1&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for&amp;nbsp;alcohol dehydrogenase 1&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="15" name="ori" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5735-6323" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;high-copy-number ColE1/pMB1/pBR322/pUC origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="14" name="LEU2 promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4735-5140" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;S. cerevisiae LEU2&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="13" name="GAL4 activation domain" directionality="1" translationMW="12396.26" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" consecutiveNumberingStartsFrom="768" maxRunOn="2937" maxFusedRunOn="2937"><Segment range="1558-1899" color="#993366" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;Saccharomyces cerevisiae GAL4&lt;/i&gt;&amp;nbsp;(truncated)&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;activation domain of the GAL4 transcriptional activator&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="12" name="ADH1 terminator" type="terminator" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3336-3523" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;S. cerevisiae ADH1&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;transcription terminator for the &lt;i&gt;S. cerevisiae&lt;/i&gt; alcohol dehydrogenase 1 (&lt;i&gt;ADH1&lt;/i&gt;) gene&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="11" name="AmpR promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7355-7459" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="10" name="loxP" directionality="2" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5351-5384" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;Cre recombinase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;Cre-mediated recombination occurs in the 8-bp core sequence (GCATACAT).&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="9" name="loxP" directionality="2" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5462-5495" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;Cre recombinase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;Cre-mediated recombination occurs in the 8-bp core sequence (GCATACAT).&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="8" name="lac promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment name="-10" range="5230-5236" color="#ffffff" type="standard"/><Segment range="5237-5254" color="#ffffff" type="standard"/><Segment name="-35" range="5255-5260" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for the &lt;i&gt;E. coli lac&lt;/i&gt; operon&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="7" name="HA" directionality="1" translationMW="1102.17" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="2937" maxFusedRunOn="2937" detectionMode="exactProteinMatch"><Segment range="1942-1968" color="#cc99b2" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;HA (human influenza hemagglutinin) epitope tag&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="6" name="CAP binding site" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5275-5296" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;E. coli&lt;/i&gt; catabolite activator protein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;CAP binding activates transcription in the presence of cAMP.&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="5" name="SV40 NLS" directionality="1" translationMW="883.15" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" consecutiveNumberingStartsFrom="126" maxRunOn="2937" maxFusedRunOn="2937" detectionMode="exactProteinMatch"><Segment range="1522-1542" color="#993366" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="locus_tag"><V text="&lt;html&gt;&lt;body&gt;&lt;br&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;&lt;br&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;nuclear localization signal of SV40 (simian virus 40) large T antigen&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="protein_id"><V text="&lt;html&gt;&lt;body&gt;&lt;br&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="4" name="T7 promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1905-1923" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for bacteriophage T7 RNA polymerase&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="3" name="M13 rev" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5182-5198" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="2" name="lac operator" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5206-5222" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;lac&lt;/i&gt; repressor encoded by &lt;i&gt;lacI&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;The &lt;i&gt;lac&lt;/i&gt; repressor binds to the &lt;i&gt;lac&lt;/i&gt; operator to inhibit transcription in &lt;i&gt;E. coli&lt;/i&gt;. This inhibition can be relieved by adding lactose or isopropyl-Î²-D-thiogalactopyranoside (IPTG).&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="1" name="Kozak sequence" type="regulatory" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1930-1939" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;vertebrate consensus sequence for strong initiation of translation (&lt;a href=&quot;https://www.ncbi.nlm.nih.gov/pubmed/3313277&quot; title=&quot;https://www.ncbi.nlm.nih.gov/pubmed/3313277&quot;&gt;Kozak, 1987&lt;/a&gt;)&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="regulatory_class"><V predef="other"/></Q></Feature><Feature recentID="0" name="TYMS" directionality="1" translationMW="35716.05" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="1996-2937" color="#ffcc00" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature></Features>
   —<?xml version="1.0"?><Primers nextValidID="0"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/></Primers>
  A<Notes>
<UUID>18a940d4-24e9-422a-98bb-d398fbbbb0ff</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="7:22:23">2018.6.14</Created>
<LastModified UTC="2:16:40">2024.8.13</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  e<StrandColors><TopStrand><ColorRange range="0..2931" colors="black"/><ColorRange range="2932..2943" colors="green"/><ColorRange range="2944..8907" colors="black"/></TopStrand><BottomStrand><ColorRange range="0..2931" colors="black"/><ColorRange range="2932..2943" colors="green"/><ColorRange range="2944..8907" colors="black"/></BottomStrand></StrandColors>  2�      ç  4        4���¼Å†	XVA[PM…TufP,\UeHU"†a‰¨:Œ3ÅUÄ©ÍhÝmŽâÌî�Ãs€ÈCˆü0Jª3ãj½B§øÎPF�ÖQcÂ€(Æq÷x"ÚjR�á#j5ØIžEÔz1Ä¨ð‚1u‘1ö¡BÌÙ|äIêA]5�(çÒedt'g]àV
.*s«ZÚ€ˆÞ¦˜§Y�@^§zï˜Ý™¥¥KÖ9$E 	wþó;ªñª«•òš°fú¢Š¼�f¯º®}_ÙÛ¯™gë(.þ]‰0@™ÖEÑŸGQÙ`9]G�õ§xûh`‚v\^YâOäz=¶Ù±J·Ÿê¯j=¹¡Ie�Í�zêºª«ªîïü¢‹·Ù¼ÖUVw•U¥ÇTtß]²ŠôN@&ìSwê¯¯­­ÍÞ{¹a5Âg¯¯Jz¡YUT¨âèæÔ™Y�i¥º¢¯²Ú+²¿«¯¢®ªãušµ9ŸSg[^õ:�Q�•0ZZüO½UEP±h	óÏZ¥µHš8dí¢úÞ­åÒMp¼_¶]{WV»S:·_Z>û—+a—­…–·IV†%–µ_x·à p}^2eÌ¨Þt*Y÷�`a‰™y¹¬a…ÿ}_ÕUº–qíæ@ÄÏ=�'öqÉôiÎÙƒØô…¯lg½±;�[$g…WüÔ­ë^#l�öíjêÚ™Û*�®©‰yžû”«[ ö%û†æEª¥aäZXýºª ~VºØU™§:kE:�ë¬ôB µ.õµ
,:.~�EjqT`UÕ²‚FrŠë•”Å«¦˜j¦šVR™UVR&uj•!î�ªUÅZé®„—º¾$-
:»BdØBÎé¡÷~çÃÂÅkþ˜]šö¨–¬‹*æ˜Z¶¦T©‰Õ›eUD[ª”Ô®—®°ÇM·Ç¬ ž(ß•·Ò¸gãoUú©xÛ­`ú~ºÐÔ­aÖ­vÚ4ÑÔdƒ‡é® Õ¸Eò‚²u}XÂ+¢àÕõ4`×Wõø\ël†mõv(à†™gÚ�€ +¥ä`ÃEµµ¡ŠëX�V²º*ûÝ*®ö[¡D=Wi:›ÔVCX íŠ$aSk¢ê7®Â&ŠÞoÔÇ}·Û.¾®éõIYû÷–»‡]qKIú“Vˆ:e.U,Õ)Ø.” ÉÖ…+©Áª’Wè­—yøŸö¤tß—UÃm~º:ôÏ¶àRß‰2ÆM7ßZV¬VÅèv�qvÑY:”_¥ƒ¬8†®‡× ·àÕqÆë¤Gi	›èVfffŽ‰T%JEIG9}ù U]d/¥eš-zÔš!�M©-…Î»šæaTð¯îß«ê®ýj­(UBf2ÐÇî¥ÕV¯KlZ†¸ŒdÏøˆ®Êb„U–
9Dkº6éÆ~k÷J nÇ¹9mk%¶dâ¯ˆ…h¦ó·Z÷uÙÄðSeH•> �–—·ihÊ3·�Î¯K_‹#Ó†^²e‰Žg¦�¸Q©ËÓÔäè8Ð±0óÁ¥™zÉ–&9œR›N6–Ã×3 D0¨É 3ºó¯³·‡³Œì,¬8Ã	´ííPÂYÂ›ÝÐ\ÐT]ˆMT=5PôÉU2Žª½�ï?þM·Ulsµ-ƒüteF}«ÿ�NþÈÎïŠñþ<éðgâóêïëLƒ:»díÎ$&í+�²³½Nˆ�Åÿò´ü|ã>›Â^lºõü%Ç¦Ïx4ÓÞ	tM˜�Â9Ñ+¡H ùyÃ�äPtš‚n3¸áOJ~°UD “ºÃWWß8$Â
ªŠ�M4iÌÑUPnÔQ¾¡º®p‡;H{r”¨-„iµT­ùê•¥VªVÖåI™„•è.)õŽ–Òß›´Æâæ¬­½’�‡¿Ë"F^qœŸnôŽ5†|Äs·Ã½›Me}=<4Rã^¦óïoKšZ(ðäII+=)d¨ÍrŠ=£�²·;,¨&¶´ï
ýqµJvÿbˆÕTÂ“¾°¾ñ²‹E%(<Ò)·¶
`ÓxÙkºx¶=¼”cªâIí}Kk„Y"c|J;£ðËYÀeŸ3Ý“EêU#¶Ú\®°UîÔ§Î(Fô¬b_ÞÈß×†ëMº§«}2®Ut5XóUPP1ÜŠ{gºú«Y¦…6¦Ð)¢í„Á�š…‰©Y]Y ¯3v¯ñT�~¹E}¦›aÚ_™U�É·þ¬»3*Âp…_¸wcNüÂÝ:.%0cÀMOË@Ì´ÀŠ°—˜ÃmF‰á7ÙsjSew7˜òºí‰™™�w4fB1È]!8˜ŒÓ³(Ú¬ÎÊ¬Î¡Jù¯¯šîNEI�^´Lê…_U3¸QÍêÕWÕ\ß‰q9åY£|ëÝ]£µRnê9™»ˆæoæeùý½ú³¾…èÛ•ì~çj©Š›¹&žêGUV‰%šÙÅ]gbWjùœ’fDuYWÊÙj¸}aE¶×9·«ŠÝRc5½Û	‘f[ cên…U�üÓ¬ä–ÒW0�-ª¦VG}RO®¹~—çHÕR›Q6™7TUR[”c¨¤UdÎì6ª$]UE&FºŠ	VLîÌ£jâ¸•XÓ´k¨¨Ui3»2�ªŠ§UaMÑ®²‚U“:³(Ú¸®%V4íê*ZLîÌ£j¢¹ÕaMÑŽ²Ž      3   -<AlignableSequences trimStringency="Medium"/>  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  'a<?xml version="1.0"?><Features nextValidID="19"><Feature recentID="18" name="Kozak sequence" type="regulatory" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1930-1939" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;vertebrate consensus sequence for strong initiation of translation (&lt;a href=&quot;https://www.ncbi.nlm.nih.gov/pubmed/3313277&quot; title=&quot;https://www.ncbi.nlm.nih.gov/pubmed/3313277&quot;&gt;Kozak, 1987&lt;/a&gt;)&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="regulatory_class"><V predef="other"/></Q></Feature><Feature recentID="17" name="lac operator" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4286-4302" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;lac&lt;/i&gt; repressor encoded by &lt;i&gt;lacI&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;The &lt;i&gt;lac&lt;/i&gt; repressor binds to the &lt;i&gt;lac&lt;/i&gt; operator to inhibit transcription in &lt;i&gt;E. coli&lt;/i&gt;. This inhibition can be relieved by adding lactose or isopropyl-Î²-D-thiogalactopyranoside (IPTG).&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="16" name="M13 rev" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4262-4278" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="15" name="T7 promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1905-1923" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for bacteriophage T7 RNA polymerase&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="14" name="SV40 NLS" directionality="1" translationMW="883.15" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" consecutiveNumberingStartsFrom="126" maxRunOn="2052" maxFusedRunOn="2052" detectionMode="exactProteinMatch"><Segment range="1522-1542" color="#993366" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="locus_tag"><V text="&lt;html&gt;&lt;body&gt;&lt;br&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;&lt;br&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;nuclear localization signal of SV40 (simian virus 40) large T antigen&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="protein_id"><V text="&lt;html&gt;&lt;body&gt;&lt;br&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="13" name="CAP binding site" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4355-4376" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;E. coli&lt;/i&gt; catabolite activator protein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;CAP binding activates transcription in the presence of cAMP.&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="12" name="HA" directionality="1" translationMW="1102.21" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="2052" maxFusedRunOn="2052" detectionMode="exactProteinMatch"><Segment range="1942-1968" color="#cc99b2" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;HA (human influenza hemagglutinin) epitope tag&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="11" name="lac promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment name="-10" range="4310-4316" color="#ffffff" type="standard"/><Segment range="4317-4334" color="#ffffff" type="standard"/><Segment name="-35" range="4335-4340" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for the &lt;i&gt;E. coli lac&lt;/i&gt; operon&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="10" name="loxP" directionality="2" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4542-4575" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;Cre recombinase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;Cre-mediated recombination occurs in the 8-bp core sequence (GCATACAT).&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="9" name="loxP" directionality="2" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4431-4464" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;Cre recombinase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;Cre-mediated recombination occurs in the 8-bp core sequence (GCATACAT).&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="8" name="AmpR promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6435-6539" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="7" name="ADH1 terminator" type="terminator" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2416-2603" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;S. cerevisiae ADH1&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;transcription terminator for the &lt;i&gt;S. cerevisiae&lt;/i&gt; alcohol dehydrogenase 1 (&lt;i&gt;ADH1&lt;/i&gt;) gene&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="6" name="GAL4 activation domain" directionality="1" translationMW="12396.64" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" consecutiveNumberingStartsFrom="768" maxRunOn="2052" maxFusedRunOn="2052"><Segment range="1558-1899" color="#993366" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;Saccharomyces cerevisiae GAL4&lt;/i&gt;&amp;nbsp;(truncated)&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;activation domain of the GAL4 transcriptional activator&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="5" name="LEU2 promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3815-4220" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;S. cerevisiae LEU2&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="4" name="ori" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4815-5403" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;high-copy-number ColE1/pMB1/pBR322/pUC origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="3" name="ADH1 promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="771-1476" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;S. cerevisiae ADH1&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for&amp;nbsp;alcohol dehydrogenase 1&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="2" name="AmpR" directionality="2" translationMW="31558.16" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="6365" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1" detectionMode="exactProteinMatch"><Segment range="5574-6365" color="#ccffcc" type="standard" translated="1"/><Segment name="signal sequence" range="6366-6434" color="#ccffcc" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to ampicillin, carbenicillin, and related antibiotics&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;Î²-lactamase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="1" name="LEU2" directionality="2" translationMW="38982.00" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="2720-3814" color="#ff7f50" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;S. cerevisiae LEU2&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;yeast auxotrophic marker&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;3-isopropylmalate dehydrogenase, required for leucine biosynthesis&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="0" name="2Î¼ ori" type="rep_origin" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6821-7985" color="#ffff00" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;yeast 2Î¼ plasmid origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature></Features>
   —<?xml version="1.0"?><Primers nextValidID="0"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/></Primers>
  @<Notes>
<UUID>18a940d4-24e9-422a-98bb-d398fbbbb0ff</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="7:22:23">2018.6.14</Created>
<LastModified UTC="2:12:3">2024.8.13</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  ˆý7zXZ  æÖ´F !   t/å£àT@F] Ë‡ØÎf‘ƒÊý{3Ôé·Ú(1v%f M*	mj÷)p80çÌ*iÚ	´NY]Qó“�úF7î&ƒÕ!Ò÷ÀnÔúéåŸ~éÙ¬ÑMU¾qé÷ƒA�uÑo)÷BH™Ö€#¿€³ùu;*•b(q
wYwc ¿§èc7‹XÑiÚ”VÌ¢¤Ékk[~ª’eñ¬¡þïg\²›³­¶\N·$QäçŠÊŸSçe†Ì¸à_áœ¯Î?-#^>”²Òþ8wÇþÎÍì”�–›SÿûCì.#-»?&ì˜óÎ²Zù<›¨ª¥ 9âyFÕ;i–�Ì»L(¼}^•Š=«Ú¢-f	‹ò¢�ßfPê—AòAE‡LuÔ§ÏÆÜ&ðÐk©­M•P‹œ·vÊÌ¿ËtP¯šª�_€wbå;˜×À“Ã‡]Eá;ï£¬z‡![TÄÂ×�^ÐÐë ûr]ÀÆ¢Ê*Þv¬¥z
¡TTbƒ¿Ô£\�ƒÃ0±¦v;Ö¾+
VÝòàøÖrxiêq	–­«wr2Þ”ÔðÂdDÅkZÄ,f!6â}*túœÉô-�–iT‡\6vÙ�‹‹œÒxžÙå–HNFKwÐk¡|­}NpWÈ»6z4þi¬8YâYÈn*†ÞãªæZ¾{d‘6Ó{.Æ™â³bÅ³Î7ÿHU g›ü_&ø#D­Â_Ÿ�y2ˆt§Ë¸$Æä&LÙO8{Ä Å<}�¬œ3•«î¨jöÐ�FòIŒ¥ÿ~9!ó�Éhâ]g®,[…íéÓ¿y%p48y©
©pdC/H¸¸-!w’†zxÛãTŒš[.'èØLÄ¿›àÃ—Ý¼GFtÁœc½,0)ÞˆË,–zèÕÈõfÏE¸‚Ø±A]¡®·?
µqK/‡+[l}yóm_~’„Ùû©i@`Mÿþîû8ø²\>h6²4¯Lžê[¹h?…ü"4¬53Fh�º~RÎÝ¦¸ä„rÕxøÅyöÖ•k3->÷•Ã|"^*xu K¿,·ˆG ×•¢(2C“Ô¹m¡·òX>•ªŽVÃÛcôÊ�itÎ!î’iùZó9	ã`z÷À©)ÑÃnÏÍÝ²ˆ+™¸%ý^Z‚ÚE¬ú{cÔ{¾l4gTŸRì®tlÒFJ§5Ô��™³bt¬ò‡¸{‚3D‡‡I�ã¼‘’7�RÈô8îW¥å:lm±ÓY3¾û¬þ•Lt>¥Ï[þuz²¬‚4¡Pz€f�Ëpú-4Ÿ¦e2j"¶ÿœ—¤²€iJ¼ñö£T�±.whž;®‘¯Ù¿“eÊª)ß»Â¸0w§päü\ƒ¤‘ÈûAQÅ0iPÚz¨ò•À6°r-¿&o„Ã?GÚñU�ìIMCÅpY@~VÏ�Ëd—wÓ¼A!4×ªâfeI >¦õõx'cU-C¡™K[ëV˜Í˜½ˆ.ì³²Ês¯Øã»°�ê‡ÞCr
U¢Ÿ¨’ÔÅr§±O ÕŸ5w©¡„7¤(>Ÿ¬?¶eÈ˜WGBœyÒ€¼²¼ßÌÌþ)<z�{ÒGæ-{Ò�,öz†Íß�mz hù(nöˆtqàN»Ù—ÕˆI;Æî±,7ìÐ.èÚŠ\ ö¿*‰(©6áR†Ò†`!ïËÌ}Î`ðÅVH'l´ÿ•£(dÿÓã�]f‘¥‡-PEmÔ¾¢ 0µ¸%AL«¡È·Ñë®§¢Ò„7º.0¸Ä¹C•œÝ{>¼*Œ0¼Lðõ*üÆ%#ø¦]h„Á¢½èÍ"Þ]+øËSPì
orð@Æ%º�{H’±¨ „<--5ê;ØöµÚ!cOÚ[O4ÝØÔÏ‘r¡‡�‘yxŸÖ$ Z¦±ßôL-�ö_!‡”Í•  ìñðáÃ:ë8Žù.ÒCé£B~ì„ƒg£çÊudzÜ/ÞŒ³äx¶Ê $Ûè¯ãŽ¨þ/ÛúóªJÇ‡¯ôy@.¯‚(Å»êm§•¹H(ýu´ˆoK”ø?q’³aÈ=YÕ|Óo6C"xâÏïÒ¤�'À-vPüÕ¯K š‹5û·Sêë.•ªgnXÀƒË¹¥©Õ{¨Ä§qÜ6�©õ¼²"Öüh¹|ÌöŸõµlåêZa‹»Í¡ïW±¸œö;´ªÿ7+ø•ý$á'’ïUâ¡q„¢„ñ èbß`�Ôuóþ_Wò¥3CºÛg§]6îž³À%7®AÛ9€Ìqqµ‰Ù¤GÊ¼Þ‰ÜNŽÙõœ°(¬KSH\GË�Ã¨ƒª%¢5ù6YÂ´¡†]­™*ís(.LÆð—Õ;…@>jÿbýÐI®Ðî�ÙqØ,2ø¦ØzWŠÑF�^e€øx¾©Çÿ{0-¶ �À€zhEþZ„‘(@äH?,+›ý)G—Äç,šŠ(FÝ¿o]kW÷”ƒõ)‡0I§’bLþ‰7»×�²„òm«Hø�Íœã,èIŸ_{+Ûz„KƒK�‰7»ü¦ì“Áw¬ò7t™Æ7a#fÔœøtàvë¤ŠZåè›Ím„ÛZlÓÑ÷Ö#ºwW»4;­tÏÊ&×yÍvøêáÅ&öN*�\¤þFÙÁØ„ûjf,i/½åd÷¥üá �ì0¼½|Ñ K‰íÞ°8~›wI·W�,Ýç‡:€Lúð‹•²%HØc�šò±sè©™š‘ú>ðòÜ"ÃgJ+–ÌòX7²ÑUšB§Xxžüæ=Œ¤+Ìp¼é?ÛeL§B² ó
’Ðý%¼º©êö³‘3¬Þ�ºÕ¯ë`k!£•»‹¹^C’K1¡/~É�“Ü¬tsŒp�³§xÝ�Ê\ªÛPÙ¡ÏæëÔ½ƒá(Š>‰•gm†¡{lP)q´ö"KZ|å² mÙ°«àc¶¥Ë–íý=Midêæöu‚+'fr´xhe¥~ c¿zÿ;ûñMeM¸tñ,"âØè,‡|‚qþc ¶t.ü‰×�ÍÜíj�
�&vŠL1®Àœdèb
j[Ïyu¢’^‘Q:-™”ü<ü·àÃú_uƒŒíøÕi«ó±ýþ½ú,rµ�²÷¶éN�Æ€¨\ªÈ¥l—½—•©ÎaíÁC]Èéº‡ñGG¿‰>±&ø/ÜûK§:j`¼Ì’ðÐ…X
¦ËTÅì{oaÚÒ�?é¶ò9Ó;éË":¬¢CEuÆñÓè¶|æÓzu<ZZç>ìÇ¡|ÉßÑž/ÚJf½ Ÿ¤öQ1êÏ…ØxUŠµO·xQ´cšºAšú$DëÀòEö7–¢¼¼SQ*ºýÙ¾Ð>z ~‰Ïf¶Q;~™ÿ¾®w8U˜nóù¡s›{~wayñîÄÒü¥²6 ?à!™vüîJÉ²$”¬OkNŠl6î×[pIY"wR‹ËJ·³¨e;ÄŒÈ¶Ø,M©œ'©†(‰•ç›3 Pœ�WŸÃç2,7ýèël¼áÄ(j.VäÃ±Oòª@•ç>beMâ¿>Øöèg„fy}ã'ëâñó”ïèÞÁrÔ¸sÍ„@õÇ°Óíè9Ó+ÚÄLø[î¨iÏŒÑnk*–-ºW%.k[Z J]�7GÓ¿EÁ�kœd®<q\¸µŽhØ ”Å¦m£2H)#oM ¨£ë21Òe‚õ&ì6Ì¤0ô1©€?šmòOéº­¶Mr%Ù‡²ŠY}$Š/’q%¾wl,ê•9"³�cºð‰¢þQ¤âðÌÊç/è÷#›•zQ¥{U3ì&}:    )òq'Ph°ô âÁ¨ Œ¸øw±Ägû    YZ   -<AlignableSequences trimStringency="Medium"/>  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  /<?xml version="1.0"?><Features nextValidID="20"><Feature recentID="0" name="MCM5" directionality="1" translationMW="82285.74" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="1996-4200" color="#ffcc00" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="MSGFDDPGIFYSDSFGGDAQADEGQARKSQLQRRFKEFLRQYRVGTDRTGFTFKYRDELKRHYNLGEYWIEVEMEDLASFDEDLADYLYKQPAEHLQLLEEAAKEVADEVTRPRPSGEEVLQDIQVMLKSDASPSSIRSLKSDMMSHLVKIPGIIIAASAVRAKATRISIQCRSCRNTLTNIAMRPGLEGYALPRKCNTDQAGRPKCPLDPYFIMPDKCKCVDFQTLKLQELPDAVPHGEMPRHMQLYCDRYLCDKVVPGNRVTIMGIYSIKKFGLTTSRGRDRVGVGIRSSYIRVLGIQVDTDGSGRSFAGAVSPQEEEEFRRLAALPNVYEVISKSIAPSIFGGTDMKKAIACLLFGGSRKRLPDGLTRRGDINLLMLGDPGTAKSQLLKFVEKCSPIGVYTSGKGSSAAGLTASVMRDPSSRNFIMEGGAMVLADGGVVCIDEFDKMREDDRVAIHEAMEQQTISIAKAGITTTLNSRCSVLAAANSVFGRWDETKGEDNIDFMPTILSRFDMIFIVKDEHNEERDVMLAKHVITLHVSALTQTQAVEGEIDLAKLKKFIAYCRVKCGPRLSAEAAEKLKNRYIIMRSGARQHERDSDRRSSIPITVRQLEAIVRIAEALSKMKLQPFATEADVEEALRLFQVSTLDAALSGTLSGVEGFTSQEDQEMLSRIEKQLKRRFAIGSQVSEHSIIKDFTKQKYPEHAIHKVLQLMLRRGEIQHRMQRKVLYRLK*"/></Q></Feature><Feature recentID="1" name="2Î¼ ori" type="rep_origin" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="9004-10168" color="#ffff00" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;yeast 2Î¼ plasmid origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="2" name="LEU2" directionality="2" translationMW="38980.99" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="4903-5997" color="#ff7f50" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;S. cerevisiae LEU2&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;yeast auxotrophic marker&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;3-isopropylmalate dehydrogenase, required for leucine biosynthesis&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="MSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENHLIGGAAIDATGVPLPDEALEASKKVDAVLLGAVGGPKWGTGSVRPEQGLLKIRKELQLYANLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSEQYTVPEVQRITRMAAFMALQHEPPLPIWSLDKANVLASSRLWRKTVEETIKNEFPTLKVQHQLIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPDKNTAFGLYEPCHGSAPDLPKNKVNPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLDAGIRTGDLGGSNSTTEVGDAVAEEVKKILA*"/></Q></Feature><Feature recentID="3" name="AmpR" directionality="2" translationMW="31557.36" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="8548" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1" detectionMode="exactProteinMatch"><Segment range="7757-8548" color="#ccffcc" type="standard" translated="1"/><Segment name="signal sequence" range="8549-8617" color="#ccffcc" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to ampicillin, carbenicillin, and related antibiotics&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;Î²-lactamase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"/></Q></Feature><Feature recentID="4" name="ADH1 promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="771-1476" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;S. cerevisiae ADH1&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for&amp;nbsp;alcohol dehydrogenase 1&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="5" name="ori" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6998-7586" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;high-copy-number ColE1/pMB1/pBR322/pUC origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="6" name="LEU2 promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5998-6403" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;S. cerevisiae LEU2&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="7" name="GAL4 activation domain" directionality="1" translationMW="12396.26" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" consecutiveNumberingStartsFrom="768" maxRunOn="4200" maxFusedRunOn="4200"><Segment range="1558-1899" color="#993366" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;Saccharomyces cerevisiae GAL4&lt;/i&gt;&amp;nbsp;(truncated)&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;activation domain of the GAL4 transcriptional activator&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="ANFNQSGNIADSSLSFTFTNSSNGPNLITTQTNSQALSQPIASSNVHDNFMNNEITASKIDDGNNSKPLSPGWTDQTAYNAFGITTGMFNTTTMDDVYNYLFDDEDTPPNPKKE"/></Q></Feature><Feature recentID="8" name="ADH1 terminator" type="terminator" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4599-4786" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;S. cerevisiae ADH1&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;transcription terminator for the &lt;i&gt;S. cerevisiae&lt;/i&gt; alcohol dehydrogenase 1 (&lt;i&gt;ADH1&lt;/i&gt;) gene&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="9" name="AmpR promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="8618-8722" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="10" name="loxP" directionality="2" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6614-6647" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;Cre recombinase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;Cre-mediated recombination occurs in the 8-bp core sequence (GCATACAT).&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="11" name="loxP" directionality="2" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6725-6758" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;Cre recombinase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;Cre-mediated recombination occurs in the 8-bp core sequence (GCATACAT).&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="12" name="lac promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment name="-10" range="6493-6499" color="#ffffff" type="standard"/><Segment range="6500-6517" color="#ffffff" type="standard"/><Segment name="-35" range="6518-6523" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for the &lt;i&gt;E. coli lac&lt;/i&gt; operon&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="13" name="HA" directionality="1" translationMW="1102.17" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="4200" maxFusedRunOn="4200" detectionMode="exactProteinMatch"><Segment range="1942-1968" color="#cc99b2" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;HA (human influenza hemagglutinin) epitope tag&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="YPYDVPDYA"/></Q></Feature><Feature recentID="14" name="CAP binding site" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6538-6559" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;E. coli&lt;/i&gt; catabolite activator protein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;CAP binding activates transcription in the presence of cAMP.&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="15" name="SV40 NLS" directionality="1" translationMW="883.15" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" consecutiveNumberingStartsFrom="126" maxRunOn="4200" maxFusedRunOn="4200" detectionMode="exactProteinMatch"><Segment range="1522-1542" color="#993366" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="locus_tag"><V text="&lt;html&gt;&lt;body&gt;&lt;br&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;&lt;br&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;nuclear localization signal of SV40 (simian virus 40) large T antigen&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="protein_id"><V text="&lt;html&gt;&lt;body&gt;&lt;br&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="PKKKRKV"/></Q></Feature><Feature recentID="16" name="T7 promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1905-1923" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for bacteriophage T7 RNA polymerase&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="17" name="M13 rev" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6445-6461" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="18" name="lac operator" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6469-6485" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;lac&lt;/i&gt; repressor encoded by &lt;i&gt;lacI&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;The &lt;i&gt;lac&lt;/i&gt; repressor binds to the &lt;i&gt;lac&lt;/i&gt; operator to inhibit transcription in &lt;i&gt;E. coli&lt;/i&gt;. This inhibition can be relieved by adding lactose or isopropyl-Î²-D-thiogalactopyranoside (IPTG).&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="19" name="Kozak sequence" type="regulatory" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1930-1939" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;vertebrate consensus sequence for strong initiation of translation (&lt;a href=&quot;https://www.ncbi.nlm.nih.gov/pubmed/3313277&quot; title=&quot;https://www.ncbi.nlm.nih.gov/pubmed/3313277&quot;&gt;Kozak, 1987&lt;/a&gt;)&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="regulatory_class"><V predef="other"/></Q></Feature></Features>
   —<?xml version="1.0"?><Primers nextValidID="0"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/></Primers>
  ž<Notes>
<UUID>18a940d4-24e9-422a-98bb-d398fbbbb0ff</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<CustomMapLabel>pGADT7-MCM5(human)</CustomMapLabel>
<UseCustomMapLabel>1</UseCustomMapLabel>
<Created UTC="7:22:23">2018.6.14</Created>
<LastModified UTC="7:46:16">2025.7.16</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  Y     K         
?????                                                                                                       ÿ d      DO  d              E              ÿÿÿÿYô? P                                                                                                                                                             Ï<StrandColors><TopStrand><ColorRange range="0..1993" colors="black"/><ColorRange range="1994..1996" colors="green"/><ColorRange range="4197..4199" colors="light blue"/><ColorRange range="4200..10170" colors="black"/></TopStrand><BottomStrand><ColorRange range="0..1993" colors="black"/><ColorRange range="1994..1996" colors="green"/><ColorRange range="4197..4199" colors="light blue"/><ColorRange range="4200..10170" colors="black"/></BottomStrand></StrandColors>   3<?xml version="1.0"?><EnzymeVisibilities vals=""/>
   )<?xml version="1.0"?><CustomEnzymeSets/>
